[BioC] eBayes

[E Motakis, Mathematics]

Dear all,

In order to perform eBayes command in R, I should log-transfrom the raw 
data so that to be normalized. However, I am not sure if I should also 
standardize them in order to find significant expression with eb$t values. 
Is that the case?

Standardization is the correct procedure or it is enough just to subtract 
log(intensity)- row logmean (without dividing by std dev)?

The SAM procedure requires the same steps?

Thanks in advance,
Makis Motakis


----------------------
E Motakis, Mathematics
E.Motakis at bristol.ac.uk