[BioC] (no subject)

Maria Fookes mcf at sanger.ac.uk
Thu Mar 11 12:04:56 MET 2004


Hello everybody,

I have started to use the limma package for a couple of two-colour
hybridization slides RNA/RNA... can anybody tell me if I can still use
it
When I have features in the array at one spacing (controls) and other
features at another spacing? Or do I have to modify my gpr file?
(generated by genepix)

And a second issue

I want to compare the above RNA(mutant)/RNA(wild type) direct method
with the option: RNA(mutant)/gDNA plus RNA(wildtype)/gDNA . Could anyone
advice me in type of package to carry out this type of normalization?
(RNA/gDNA gives a very skewed kind of data)

Thank you very much in advance
María



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