[BioC] problem for dealing with replicated spots in slide

Yi Zou yzou1971 at netscape.net
Fri Mar 5 21:56:57 MET 2004


Hi,

I'm learning using marray package to analyze microarray data. After 
studying the tutorial  using data 'swirl', I found each spot in this 
tutorial slide stands for one gene.  My data have a little difference.  
These are 3 replicated spots in each slide.  I didn't find the comand 
dealing with replicated spots when I followed the tutorial,  So, when I 
analyzed 4 replicated slides and output the gene list using command 
sunch as "stat.bayesian" function, each gene ID in the list stands for 
only one spot in the slides instead of 3 replicated spots. I want each 
gene stands for all spot data including 3 replicated spots  in each 
slide. Can sombody give me some hints to deal with this case? If I need 
to set up some arguments in marrayLayout or I have to write some function?

My data is GenePix fata, My Gal file like this:

ATF     1
43    5
Type=GenePix ArrayList V1.0    
BlockCount=40    
BlockType = 0    
"Block1=    2600    17000    140    16    250    18    250"    
.........
"Block40=    16100    57500    140    16    250    18    250"           
     
Block    Row    Column    ID    Name
1    1    1    QHA10A21.yg.ab1    sf_1_1
1    1    2    QHA10A21.yg.ab1    sf_1_1
1    1    3    QHA10A21.yg.ab1    sf_1_1
1    1    4    QHA10P20.yg.ab1    sf_1_3
1    1    5    QHA10P20.yg.ab1    sf_1_3
1    1    6    QHA10P20.yg.ab1    sf_1_3
1    1    7    QHA11H15.yg.ab1    sf_1_5
1    1    8    QHA11H15.yg.ab1    sf_1_5
1    1    9    QHA11H15.yg.ab1    sf_1_5
1    1    10    QHA11O07.yg.ab1    sf_1_7
1    1    11    QHA11O07.yg.ab1    sf_1_7
1    1    12    QHA11O07.yg.ab1    sf_1_7
...........

Thanks

Yi



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