[BioC] problem for dealing with replicated spots in slide
Yi Zou
yzou1971 at netscape.net
Fri Mar 5 21:56:57 MET 2004
Hi,
I'm learning using marray package to analyze microarray data. After
studying the tutorial using data 'swirl', I found each spot in this
tutorial slide stands for one gene. My data have a little difference.
These are 3 replicated spots in each slide. I didn't find the comand
dealing with replicated spots when I followed the tutorial, So, when I
analyzed 4 replicated slides and output the gene list using command
sunch as "stat.bayesian" function, each gene ID in the list stands for
only one spot in the slides instead of 3 replicated spots. I want each
gene stands for all spot data including 3 replicated spots in each
slide. Can sombody give me some hints to deal with this case? If I need
to set up some arguments in marrayLayout or I have to write some function?
My data is GenePix fata, My Gal file like this:
ATF 1
43 5
Type=GenePix ArrayList V1.0
BlockCount=40
BlockType = 0
"Block1= 2600 17000 140 16 250 18 250"
.........
"Block40= 16100 57500 140 16 250 18 250"
Block Row Column ID Name
1 1 1 QHA10A21.yg.ab1 sf_1_1
1 1 2 QHA10A21.yg.ab1 sf_1_1
1 1 3 QHA10A21.yg.ab1 sf_1_1
1 1 4 QHA10P20.yg.ab1 sf_1_3
1 1 5 QHA10P20.yg.ab1 sf_1_3
1 1 6 QHA10P20.yg.ab1 sf_1_3
1 1 7 QHA11H15.yg.ab1 sf_1_5
1 1 8 QHA11H15.yg.ab1 sf_1_5
1 1 9 QHA11H15.yg.ab1 sf_1_5
1 1 10 QHA11O07.yg.ab1 sf_1_7
1 1 11 QHA11O07.yg.ab1 sf_1_7
1 1 12 QHA11O07.yg.ab1 sf_1_7
...........
Thanks
Yi
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