[BioC] How to modify an Affybatch object?

[Puhong Gao]

Got it! Many thanks to Rafael and Johannes for their help.

Best,

Puhong

----- Original Message ----- 
From: "Rafael A. Irizarry" <ririzarr at jhsph.edu>
To: "Puhong Gao" <pgao at hunter.cuny.edu>
Cc: <bioconductor at stat.math.ethz.ch>
Sent: Monday, March 01, 2004 1:28 PM
Subject: Re: [BioC] How to modify an Affybatch object?


> you can use the pm method, e.g to put the mean of the pms in the first
> column (array) use:
>
> pm(abatch)[,1] <- rowMeans(pm(abatch))
>
> On Mon, 1 Mar 2004, Puhong Gao wrote:
>
> > Hi, all,
> >
> > Is it possible to insert the modified PM values back to the original
affybatch and then to normalize it?  I'd like to use the averaged
intensities of my technical replicates to normalize against other biological
replicates. Any comments and/or suggestions will be very appreciated. Thank
you.
> >
> > Puhong Gao, PhD
> > Dev. Psychobiology
> > NYSPI, Unit# 40
> > 1051 Riverside Dr.
> > New York, NY 10032
> > (212) 543-5710
> > [[alternative HTML version deleted]]
> >
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>
>