[BioC] Limma:What's wrong with my Targets file?
xpzhang at genetics.ac.cn
Mon Jun 14 10:13:00 CEST 2004
I am trying to do single-channel normalization for my serverl slides
using limma. After I read the userguide of limma, I made a Targets
file.It is just like:
SlideNumber FileName Cy3 Cy5
1 2004-4-2-cy3(450)-0409-Con-only532.gpr Con Blank
2 2004-4-2-cy3(450)-0410-A24h-only532.gpr A1h Blank
3 2004-4-2-cy3(450)-4526-A5h-only532.gpr A5h Blank
4 2004-4-2-cy3(450)-4527-A10h-only532.gpr A10h Blank
5 2004-4-2-cy3(450)-4529-A1h-only532.gpr A24h Blank
And the file's name is Targets.I put it with the five *.gpr under the
same directory of limma's data.
But I failed in running limma.This is the result:
> targets <- readTargets()
Error in file(file, "r") : unable to open connection
In addition: Warning message:
cannot open file `Targets.txt'
Could anyone tell me why?
Thank you very much!
Xiaopeng ZHANG <xpzhang at genetics.ac.cn>
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