[BioC] AffyRNAdeg slopes

[Lawrence-Paul Petalidis]

Hi, 
I believe the consensus on this is that it is more important to have
samples with comparable slopes than it is to actually quantitatively
assess the slope itself. I have worked with clinical samples that gave
me rather steep curves but the data was very good. Its just good to see
that you don't have any samples with curves that are way out of what the
others are doing.

Yours, Lawrence

_______________________________
Lawrence-Paul Petalidis
Ph.D Candidate
 
Department of Pathology
Division of Molecular Histopathology
University of Cambridge
_______________________________

-----Original Message-----
From: bioconductor-bounces at stat.math.ethz.ch
[mailto:bioconductor-bounces at stat.math.ethz.ch] On Behalf Of David
Carter
Sent: 08 June 2004 18:46
To: Bioconductor list serv
Subject: [BioC] AffyRNAdeg slopes

Hello everyone,
I was wondering what are acceptable values for the slopes generated 
using summaryAffyRNAdeg in the affy package.  I assume 0.0 would be the 
ideal, but what values would be considered 'of concern', or too high?  
Any thoughts from users of the RNA deg function?
Thanks.

-- 
David Carter
London Regional Genomics Centre
Robarts Research Institute, Room 4.01
PO Box 5015, 100 Perth Drive
London, Ontario, Canada, N6A 5K8

phone:	(519) 663-3253
fax:	(519) 663-3037

dcarter at robarts.ca
http://www.lrgc.ca/

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