[BioC] Normalization with cDNA microarray Control Spot / limma

[Sek Won Kong,M.D]

Hi,

 

We made custom cDNA microarray, and put 20 control spot out of 360 total
features in a single slide.

I'd like to know whether limma has a normalization function that uses
control spots. If yes, how to define control spots in limma? If no, is there
any other package that can handle normalization with control spots?

I want to make MvA plot with control spots on straight line.

 

Thank you for the helps in advance.

 

-Sek Won


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