[BioC] VSN combined with RMA
Remo Sanges
sanges at biogem.it
Mon Jul 19 22:56:39 CEST 2004
I' m planning to test vsn normalization on affymetrix arrays
with RMA background correction.
But from the vsn vignette and other messages in this list
it seems to me that vsn on affymetrix chips is used without
background correction:
e=expresso(data,bg.correct=FALSE,normalize.method="vsn",pmcorrect.method
="pmonly",summary.method="medianpolish")
There is a reason for this?
Is it wrong to use expresso in this way:
e=expresso(data,bg.correct="rma",normalize.method="vsn",pmcorrect.method
="pmonly",summary.method="medianpolish") ?
Thank you
Remo
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