[BioC] marray or rma?
Adaikalavan Ramasamy
ramasa01 at cancer.org.uk
Sun Feb 22 00:58:37 MET 2004
'marray' is a package designed for spotted two color arrays.
rma is a function in the 'affy' package which is designed for Affymetrix
chips. If your data is affymetrix, then you need the CEL files and
something along this line :
setwd("/path/to/cel/files")
# change working dir OR you can use the option filenames in ReadAffy()
raw <- ReadAffy()
out <- rma(raw)
expressionValues <- exprs(out)
write.table( expressionValues, file="out.txt", sep="\t", quote=FALSE)
This will get you the expression values in a matrix format.
Regards, Adai.
On Sat, 21 Feb 2004 gjam at daimi.au.dk wrote:
> Hi,
> I don't know the difference between the use of the 'marray' package and the use
> of the function 'rma'. I would like to calculate the expression from raw data
> and to normalize all the data afterward.
> Thanks for your help,
> Jamila
>
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