[BioC] get exprs from AffyBatch
Rafael A. Irizarry
ririzarr at jhsph.edu
Wed Feb 4 17:17:34 MET 2004
i would like to add to jim's suggestion that
there is also
exprs2excel (myAffyBatch)
for creation of excel compatible files.
On Wed, 4 Feb 2004, James MacDonald wrote:
> I'm not exactly sure what you are trying to do here, but I am going to
> assume that you want to compute expression values for your genes, and
> then export to a file.
>
> The AffyBatch contains your raw PM and MM data, not expression values.
> When you do
>
> exp <- exprs(myAffyBatch)
>
> You are simply creating a matrix of PM and MM values. To compute
> expression values, you want to do something like
>
> eset <- rma(myAffyBatch)
>
> see also ?expresso and ?mas5 for other ways to compute expression
> values. Once you have run rma or whatever, you have an exprSet that can
> be used with write.exprs() to export.
>
> Note that the function you want is write.exprs() not exprs.write(). In
> addition, you only have to pass the variable name of your exprSet and
> the filename. I don't know if you actually used file=** and sep=*, but
> you don't need sep (default is "\t", which is fine for most uses), and
> file=** will give you a syntax error. You need something like
> file="Expression values.txt"
>
> HTH,
>
> Jim
>
>
>
> James W. MacDonald
> Affymetrix and cDNA Microarray Core
> University of Michigan Cancer Center
> 1500 E. Medical Center Drive
> 7410 CCGC
> Ann Arbor MI 48109
> 734-647-5623
>
> >>> Hao Liu <liuha at umdnj.edu> 02/04/04 12:29AM >>>
> I tried to use exprs to get expset by
>
> > exp <- exprs(myAffyBatch)
>
> I then tried to write:
>
> exprs.write(exp,file=**,sep=*)
>
> however, I can't do this, seems exp is not really a
> expset object.
>
>
>
> Best regards
>
> Hao Liu, Ph. D
>
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