[BioC] GCRMA/RMA bimodal distribution
Hannah at mpimp-golm.mpg.de
Wed Aug 25 16:49:10 CEST 2004
This has been mentioned before in the context of rma and that it was an
artifact of BG correction.
I was very suprised to see that gcrma also gave a very pronouned bimodal
distribution. When comparing samples, obviously the relative positions
of the 2 peaks may influence observed expression changes. Would such
peak shifts be more likely in divergent samples, and if anyone wants to
comment on those.... ;-)
This example is using 12 chips (biological reps). But I initially
noticed it using 3 and 6 chips in rma.
Hope attachment works.
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