[BioC] normalizeWithinArrays "composite"
M Inmaculada Barrasa
ibarrasa at mail.med.upenn.edu
Thu Aug 12 01:27:52 CEST 2004
Dear all,
I am trying to normalize a test array. I would like to use
normalizeWithinArrays "composite", but I tried the other options too.
When I do:
MAloessNoC <- normalizeWithinArrays(RG, method = "loess", weights=NULL)
MAloess <- normalizeWithinArrays(RG, method = "loess", weights=NULL,
controls = nonDE)
MAnone <- normalizeWithinArrays(RG, method = "none")
I don't get any errors
But when I do:
MAcomp <- normalizeWithinArrays(RG, layout, method = "composite",
weights=NULL, controls = nonDE)
I get:
Error in predLoess(object$y, object$x, newx, object$s, object$weights, :
NA/NaN/Inf in foreign function call (arg 3)
Is my problem in the layout? How can I find out exactly where?
Also, Is there any way to do a composite loess normalization using
nonDifferentiallyExpressed genes, but without using print-tip loess
normalization. (I was using a grid with 1 column and 1 row in my layout).
Thanks a lot for your help
Inma
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