[BioC] blue fuse output and limma
smyth at wehi.edu.au
Wed Aug 4 04:49:52 CEST 2004
At 04:34 AM 4/08/2004, marchiem\@libero\.it wrote:
>we are evaluating a new image analysis software called Blue Fuse that uses
>a Bayesian approach to estimate the background. The outputs per each
>channell of this software are values with the background already subtracted.
>How can I read in these output in BioC, specifically in limma?
>I've used read.maimages and I've specified the columns just for foreground
>signal and I put NULL for the background:
The recommended way to read BlueFuse data into limma is
RG <- backgroundCorrect(RG, method="none")
This question has been previously addressed on this mail list, see:
BlueFuse also has a confidence value column, although I am not sure of the
precise name of this column. (There isn't any online documentation.) To
make use of this column, you could include a 'wt.fun' argument to
read.maimages(), for example:
mywtfun <- function(x) x[,"CONFIDENCE"]
where "CONFIDENCE" is the name of the confidence value column.
>but I received this error message:
>Error in "[<-"(`*tmp*`, , i, value = structure(list(), .Names =
>character(0), row.names = c("1", :
> nothing to replace with
>I do apologies but I'm not yet confident in R so I can't understand how to
>solve the problem...
>Maybe should I create another text file with two columns more for the
>background and put zero in them?
>Does anyone know how to read this kind of data?
>Thanks in advance and sorry again if I made some trivial mistakes.
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