[BioC] subtractmm problem in "affy"
PARK,CHRISTOPHER CHANGSUN
ccpark at ucla.edu
Wed Apr 21 22:49:00 CEST 2004
Hi all,
I am trying to normalize microarray data using the subtractmm method using
the "affy" library.
The following is my R script:
set.seed(123)
library(affy)
Data <- ReadAffy()
eset<-expresso(Data,bgcorrect.method="rma",
normalize.method="quantiles",
pmcorrect.method="subtractmm",
summary.method="medianpolish")
write.exprs(eset,file="subtractmm_data.txt")
During the normalization, I encounter the following error message:
Error in if (converged) break : missing value where TRUE/FALSE needed
In addition: Warning message:
NaNs produced in: log(x, base)
...
The processed data is a grid of NA's.
Does anyone know what might be wrong here and what I could do to fix it?
Thanks again for your help,
Chris
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