[BioC] limma reading genpix files
Bryce MacIver
ibm2 at psu.edu
Thu Apr 8 00:19:36 CEST 2004
hello all limma exprts,
I have started using limma to analyse some spotted microarray data
but have run into problems trying to read the data files in.
From the limma guide, having specified the "files" , then using the command:
RG<-read.maimages(files, source="genepix")
I get:
Read 7863scan3.gpr
Error in read.table(fullname, skip = skip, header = TRUE, sep = sep,
as.is = TRUE, :
more columns than column names
I have 4 .gpr files that were generated from genepix version 3 with
7863scan3 being the first
I also tried specifying columns as mentioned in the limma guide:
RG<-read.maimages(files, columns=list(Rf="F635 Median", Gf="F532
Median", Rb="B635 Median", Gb ="532 Median"))
and got
Error in "[.data.frame"(obj, , columns$Gb) :
undefined columns selected.
I have no idea how to interpret these error messages and have to say
that my forays into BioConductor have been a frequent exercise in
frustration because of constant unintelligible error messages. Could
some one please help me in solving these issues.
I'm running R 1.8.0 on MacOS X and recently updated limma (1.3?)
thanks
Bryce
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