[BioC] dilution and spikein data

buffat laurent.buffat@it-omics.com
Wed, 22 Jan 2003 15:51:58 +0100


Hi,

Thanks for reply so fast.
I have anover question :

In my analysis of these data, I have somes "false positive" what I retreive
between differents conditions.
For example "32660_at" is "positive" in a vs b, b vs c, c vs d and m-p vs
q-t with a very good rank (8,2,2,16)

Do you thinks they also "forgot to mention it" ?

Thanks,

Laurent

-----Message d'origine-----
De : Rafael A. Irizarry [mailto:ririzarr@jhsph.edu]
Envoye : mercredi 29 janvier 2003 16:27
A : buffat
Cc : bioconductor@stat.math.ethz.ch; wfan@fhcrc.org
Objet : RE: [BioC] dilution and spikein data


good question. we are almost certain that  affy forgot to mention 2
genes that were spiked-in. more information will be made available
through the web-page

http://www.biostat.jhsph.edu/~ririzarr/affycomp.html

-rafael

On Wed, 22 Jan 2003, buffat wrote:

>
> Hi,
>
> Thank you very much for this data.
> I have juste a question :
>
> why in the Affymetrix spikein experiment, you have, in the description
file
> in your web page, 16 probes set, and in the same description file in the
> Affymetrix web site, only 14 probes set ?
> In fact, how you discovert the "33818_at" and "546_at" probes set ?
>
> Juste for information, some result of a fist analysis in the excel file.
>
> Best regards,
>
> L. Buffat
>
> -----Message d'origine-----
> De : bioconductor-admin@stat.math.ethz.ch
> [mailto:bioconductor-admin@stat.math.ethz.ch]De la part de Rafael A.
> Irizarry
> Envoye : jeudi 23 janvier 2003 23:47
> A : bioconductor@stat.math.ethz.ch
> Cc : wfan@fhcrc.org
> Objet : [BioC] dilution and spikein data
>
>
> hi!
>
> in case you are interested, the Gene Logic dilution and
> Affymetrix spikein experiment data are available along
> with R friendly description files from here:
>
> http://www.biostat.jhsph.edu/~ririzarr/affycomp/
>
> rafael
>
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