[BioC] lm.series error

[Delphine Fleury]

Dear all,
I want to analyse my affy data with limma. I have 3 genotypes A, B, C I
want to compare to the reference ref, and 3 replicates for each genotype
and ref, so 12 arrays. I have this error message I don t understand:

> data<-ReadAffy()
> eset<-rma(data)

> design
            ref A B C
Exp143hyb246   1   0   0   0
Exp143hyb247   1   1   0   0
Exp143hyb248   1   0   1   0
Exp143hyb249   1   0   0   1
Exp143hyb410   1   0   0   0
Exp143hyb411   1   0   0   0
Exp143hyb412   1   1   0   0
Exp143hyb413   1   1   0   0
Exp143hyb414   1   0   1   0
Exp143hyb415   1   0   1   0
Exp143hyb416   1   0   0   1
Exp143hyb417   1   0   0   1

> fit<-lm.series(exprs(eset), design)
Error in as.double.default(structure(list(ler = c(1, 1, 1, 1, 1, 1, 1,
:
        (list) object cannot be coerced to vector type 14

What's wrong with my design ?
Thanks

Delphine




--
==================================================================
Delphine Fleury
DEPARTMENT OF PLANT SYSTEMS BIOLOGY           Fax:32 (0)9 3313809
GHENT UNIVERSITY/VIB,    Technology Park 927, B-9052 Gent, Belgium
Vlaams Interuniversitair Instituut voor Biotechnologie         VIB
mailto:defle at psb.ugent.be                  http://www.psb.ugent.be