[Bioc-sig-seq] using DEXSeq for RNA-seq data from Linux

Martin Morgan mtmorgan at fhcrc.org
Mon Jul 25 14:13:16 CEST 2011


On 07/25/2011 02:28 AM, Jane Merlevede wrote:
> Hello,
>
> As you advise me, I installed R version 2.14 but I can't use the DEXSeq
> library: package "‘DEXSeq’ is not available (for R Under
> development)"... Do you know why?

It's a Bioconductor package, installed the Bioconductor way,

   http://bioconductor.org/install/

that is,

   source("http://bioconductor.org/biocLite.R")
   biocLite("DEXSeq")
   library(DEXSeq)

Glad the installation went ok, and I think DEXSeq will install correctly 
for you.

Usually the 'devel' branch of R is quite stable, but actually just at 
the moment there is some instability and you might experience problems 
when trying to install or load some package combinations. One error 
message is

Error in function (classes, fdef, mtable)  :
   unable to find an inherited method for function "[", for signature 
"signature"
Error: loading failed

and unfortunately the only really accessible solution, if you encounter 
this at a crucial stage of your analysis, is to wait a few days and 
update your R.

Martin

> Thanks,
> Jane
>
>
>  >R.2.14
>
> R Under development (unstable) (2011-07-24 r56492)
> Copyright (C) 2011 The R Foundation for Statistical Computing
> ISBN 3-900051-07-0
> Platform: x86_64-unknown-linux-gnu (64-bit)
>
> R est un logiciel libre livré sans AUCUNE GARANTIE.
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> Tapez 'license()' ou 'licence()' pour plus de détails.
>
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>
>  > install.packages("DEXSeq")
> --- SVP sélectionner un miroir CRAN pour cette session ---
> CRAN mirror
>
>   1: Argentina (La Plata)      2: Argentina (Mendoza)
>   3: Australia (Canberra)      4: Australia (Melbourne)
>   5: Austria                   6: Belgium
>   7: Brazil (PR)               8: Brazil (RJ)
>   9: Brazil (SP 1)            10: Brazil (SP 2)
> 11: Canada (BC)              12: Canada (NS)
> 13: Canada (ON)              14: Canada (QC 1)
> 15: Canada (QC 2)            16: Chile
> 17: China (Beijing 1)        18: China (Beijing 2)
> 19: China (Hefei)            20: China (Hong Kong)
> 21: China (Xiamen)           22: Colombia (Bogota)
> 23: Colombia (Cali)          24: Denmark
> 25: France (Toulouse)        26: France (Lyon 1)
> 27: France (Lyon 2)          28: Germany (Berlin)
> 29: Germany (Goettingen)     30: Germany (Muenchen)
> 31: Germany (Wiesbaden)      32: Greece
> 33: Indonesia                34: Iran
> 35: Ireland                  36: Italy (Milano)
> 37: Italy (Padua)            38: Italy (Palermo)
> 39: Japan (Hyogo)            40: Japan (Tsukuba)
> 41: Korea                    42: Mexico (Mexico City)
> 43: Mexico (Texcoco)         44: Netherlands (Amsterdam)
> 45: Netherlands (Utrecht)    46: New Zealand
> 47: Norway                   48: Philippines
> 49: Poland (Gdansk)          50: Poland (Oswiecim)
> 51: Poland (Wroclaw)         52: Russia
> 53: Singapore                54: Slovakia 1
> 55: Slovakia 2               56: South Africa
> 57: Spain (Madrid)           58: Sweden
> 59: Switzerland              60: Taiwan (Taichung)
> 61: Taiwan (Taipei 1)        62: Taiwan (Taipei 2)
> 63: Thailand                 64: UK (Bristol)
> 65: UK (London)              66: UK (St Andrews)
> 67: USA (AZ)                 68: USA (CA 1)
> 69: USA (CA 2)               70: USA (IA)
> 71: USA (MA)                 72: USA (MI)
> 73: USA (MO)                 74: USA (OH)
> 75: USA (OR)                 76: USA (PA 1)
> 77: USA (PA 2)               78: USA (TX 1)
> 79: USA (TX 2)               80: USA (WA 1)
> 81: USA (WA 2)
>
> Sélection : 26
> Message d'avis :
> In getDependencies(pkgs, dependencies, available, lib) :
>    package ‘DEXSeq’ is not available (for R Under development)
>  >
>
>
>
> 2011/7/24 Martin Morgan <mtmorgan at fhcrc.org <mailto:mtmorgan at fhcrc.org>>
>
>     On 07/22/2011 07:40 AM, Jane Merlevede wrote:
>
>         Hello,
>
>         I have used DESeq for gene differential expression analysis and
>         I would like
>         to use DEXSeq now for exon differential expression analysis.
>         The point is that the library DEXSeq seems to be available only
>         in R version
>         2.14.0 (unstable). I am working on Linux (CentOS), with R
>         version 2.13.0. I
>         have not found this R version 2.14.0 for Linux... Finally, I
>         cannot use
>         DEXSeq.
>         I would like to know if DEXSeq will be soon available in R
>         version 2.13.0.
>         Or is the only solution to work with Windows?
>
>
>     As Sean mentions, this requires R-devel. It is not that difficult to
>     build R from source on Linux, see sections 1 and 2 of
>
>     http://cran.fhcrc.org/doc/__manuals/R-admin.html
>     <http://cran.fhcrc.org/doc/manuals/R-admin.html>
>
>     The source code is available here
>
>     http://cran.fhcrc.org/sources.__html
>     <http://cran.fhcrc.org/sources.html>
>
>     you are looking for R-devel.tar.gz
>
>     Martin
>
>
>         Thank you for your help,
>         Jane Merlevède
>
>                 [[alternative HTML version deleted]]
>
>
>
>
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>
>
>
>     --
>     Computational Biology
>     Fred Hutchinson Cancer Research Center
>     1100 Fairview Ave. N. PO Box 19024 Seattle, WA 98109
>
>     Location: M1-B861
>     Telephone: 206 667-2793
>
>


-- 
Computational Biology
Fred Hutchinson Cancer Research Center
1100 Fairview Ave. N. PO Box 19024 Seattle, WA 98109

Location: M1-B861
Telephone: 206 667-2793



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