Thanks for the report; should be fixed in 1.22.6, which will be published
in a day or so (you need to update your Bioconductor then).

Michael


On Tue, Mar 18, 2014 at 5:38 AM, Marzia Angela Cremona <
marziaangela.cremona@polimi.it> wrote:

>  Dear Dr. Lawrence,
>
>
>  I am a Ph.D. student of Politecnico di Milano and I'm writing to you
> about the bioconductor package rtracklayer of which you are the maintainer.
>
>
>  I have a problem using the function import.bw: when I access a BigWig
> file many times, for example to import different parts of it specifying a
> range, I get the following error
>
>
>  Error in seqinfo(ranges) : UCSC library operation failed
>
> In addiction: Warning message:
>
> In seqinfo(ranges) : Too many open files
>
> Can't open
> /u/cremona/R/x86_64-redhat-linux-gnu-library/3.0/rtracklayer/tests/test.bwto read
>
>
>  Nothing changes even if I use the command closeAllConnections(). It
> seems that import.bw doesn't close the connection to the BigWig file. Do
> you have any suggestion to fix this problem?
>
>
>  Please find attached a script that produces the error and the session
> info.
>
>
>  Thank you very much for your help.
>
>
>  Regards,
>
>
>  Marzia Cremona
>
> MOX - Department of Mathematics
>
> Politecnico di Milano
>
> Milano (Italy)
>

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