Hi,

We would like to announce a new package, ReQON (Recalibrating Quality Of Nucleotides).

When a sequencing machine calls a specific nucleotide, a quality score is also provided which corresponds to the probability of a sequencing error. However, these quality scores have been shown to be inaccurate.  If these quality scores are used in downstream analyses, these inaccuracies can have a significant impact on the results.  ReQON takes as input a sorted and indexed BAM file and recalibrates the base quality scores, outputting a new BAM file with original quality scores replaced by recalibrated quality scores.  This package also provides diagnostic plots and a quantitative statistic that show the effectiveness of the recalibration on the testing set.

The package is available at http://www.bioconductor.org/packages/devel/bioc/html/ReQON.html

Any comments or suggestions would be very helpful.

Best,
Chris

--
Christopher Cabanski
PhD Candidate
Department of Statistics and Operations Research
University of North Carolina at Chapel Hill


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