[Bioc-devel] pbmc_multimodal loading issue

[Hira Jabeen]

Dear all,
I’m encountering the following error when loading the pbmc_multimodal.h5seurat file in RStudio. All required libraries are installed and loaded:
The output proceeds as expected until the following error occurs and the reference won't load. I tried to load without image and with specific assay too but nothing works:"assay = "SCT", assay.images = FALSE"

> library(Seurat)
> library(SeuratDisk)
> library(rhdf5)> reference <- LoadH5Seurat("/home/local/BICR/hjabeen/QC/Results/pbmc_multimodal.h5seurat")Validating h5Seurat fileInitializing ADT with dataAdding counts for ADTAdding variable feature information for ADTAdding miscellaneous information for ADTInitializing SCT with dataAdding counts for SCTAdding variable feature information for SCTAdding miscellaneous information for SCTAdding reduction apcaAdding cell embeddings for apcaAdding feature loadings for apcaAdding miscellaneous information for apcaAdding reduction aumapAdding cell embeddings for aumapAdding miscellaneous information for aumapAdding reduction pcaAdding cell embeddings for pcaAdding feature loadings for pcaAdding miscellaneous information for pcaAdding reduction spcaAdding cell embeddings for spcaAdding feature loadings for spcaAdding miscellaneous information for spcaAdding reduction umapAdding cell embeddings for umapAdding miscellaneous information for umapAdding reduction wnn.umapAdding cell embeddings for wnn.umapAdding miscellaneous information for wnn.umapAdding graph wknnAdding graph wsnnError in unlist(x = assays.images, index$global$images) : 'recursive' must be a length-1 vector
 
I would greatly appreciate any suggestions.
Many thanks in advance for your help!
Best regards,HiraSchool of Cancer SciencesUniversity of Glasgow, Scotland




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