[Bioc-devel] Manifest and annotation packages for EPIC methylation array v2.0

Zuguang Gu jokergoo @end|ng |rom gm@||@com
Tue Mar 21 21:35:18 CET 2023

I am aware of the AnnotationHub package, but currently I think it is not
possible to integrate with minfi.

In the last several lines in

    signature(object = "IlluminaMethylationAnnotation"),
    function(object) {
        maniString <- .getManifestString(object using annotation)
        if (!require(maniString, character.only = TRUE)) {
            stop(sprintf("cannot load manifest package %s", maniString))

Here `maniString` contains the name of a manifest package,
e.g. IlluminaHumanMethylationEPICmanifest. After loading the manifest
package, minfi directly looks for the variable with the same name as the
manifest package name. So it would be the easiest if just making a new
manifest package taking the IlluminaHumanMethylationEPICmanifest as a
template. Or minfi needs to be adjusted to allow for more flexible
integration of new manifest packages/objects.


On Tue, 21 Mar 2023 at 14:58, Kern, Lori <Lori.Shepherd using roswellpark.org>

> The maintainer for these two packages
> IlluminaHumanMethylationEPICmanifest
> IlluminaHumanMethylationEPICanno.ilm10b4.hg19
> Is actually Kasper Daniel Hansen as listed in the DESCRIPTION file not bioc
> core members.
> You could reach out to Kasper to see if there is any intention or efforts
> that have already been made to include v2.
> If not you can submit the packages. We have moved away from traditional
> annotation packages and I would suggest moving towards an annotationhub
> package submission where the data is stored elsewhere on a external server
> like S3 buckets, Azure Data Lakes, Zenodo, etc.... (we have an bioconductor
> azure data lake location that can be used;  it just cannot be store on any
> personal location like github or dropbox)
> Little modification to an existing package structure is needed. Basically
> you would remove the data to the external location,  include a metadata.csv
> file in the inst/extdata, and include the biocView AnnotationHub. More
> details can be found at
> https://bioconductor.org/packages/release/bioc/vignettes/HubPub/inst/doc/CreateAHubPackage.html
> Then submit as normal to the new package submission tracker.
> Cheers,
> Lori Shepherd - Kern
> Bioconductor Core Team
> Roswell Park Comprehensive Cancer Center
> Department of Biostatistics & Bioinformatics
> Elm & Carlton Streets
> Buffalo, New York 14263
> ------------------------------
> *From:* Bioc-devel <bioc-devel-bounces using r-project.org> on behalf of
> Zuguang Gu <jokergoo using gmail.com>
> *Sent:* Saturday, March 18, 2023 3:56 PM
> *To:* bioc-devel <bioc-devel using r-project.org>
> *Subject:* [Bioc-devel] Manifest and annotation packages for EPIC
> methylation array v2.0
> Hi Bioc-devel,
> I recently analyzed a methylation array dataset which is from EPIC array
> v2.0 (
> https://secure-web.cisco.com/1lqTYM1ya8XGMtHfnsdUpS8GBFfG2-c035Su1G2QlTqVVUgHKjstjf7gJtWM-rIZGvRTc76YuWsBkkII9eMBecVmP2e1A55mf2ahOVb9Nco1iHMg44efGw-SU_hREgUNlB7o5Sy6C-OATItf9L-NsoDSZnhzqs8WSnCWLDCRBlqDX8bOOoHRQ-xf-Ya5ki_CjTKaCuhaKmLon1QLYV-KVUrdfKTBg0FB1HEG1QPS2gNNCCpqGlOaNWCVxFitnlqZQcyJur9d80tL8Nf9Frcm66rjXthUFvP7ZBdhgy6BIvbN_rytmo9nR3tFO-vqJO7nm/https%3A%2F%2Fwww.illumina.com%2Fproducts%2Fby-type%2Fmicroarray-kits%2Finfinium-methylation-epic.html)
> and currently there is no manifest nor annotation packages for that one.
> The available packages on Bioconductor are only for EPIC array v1.0:
> - IlluminaHumanMethylationEPICmanifest
> - IlluminaHumanMethylationEPICanno.ilm10b4.hg19
> And it is not proper to use the v1 packages for the v2 data.
> I've built a manifest package and an annotation package for the EPIC array
> v2.0. They are available at:
> https://secure-web.cisco.com/1QiOF2zfvxn-NM_D59JEGrLZNxfjlhE96RB2QFStOhZ-M_vbiO6U4EAWhA4DxQl2K4UCyCR2IlwPHsInzW3sJ2AMU8ZGdfx2iG9GgacdRqfiLFso1I8byAAr3GSfV0yC6vYlWWOAcemIIiQmhz2ucGFBPnr6_XM6d-Fu8yoDbXBs4Kgr-BcAl-Pd75iP9-5p892EmkkdxAoDV7gLycQbrWXkoZ_k4OFitm-jojHDyBux8UbuaxsY6o_AM-f-5uji4V325my36EKUam9Fs_BWlQuoEm92wkvrPfLOrfSGs3gNRe_yOUL-xnZE2dgDIlwr_/https%3A%2F%2Fgithub.com%2Fjokergoo%2FIlluminaHumanMethylationEPICv2manifest
> https://secure-web.cisco.com/1cO_2hmhoc2EdgBhXjFVHzLhg8R28yb3KFaT2NGVp8p93xKzA8PkG-N2DaTdM1tL75mwBskP9Kd-h2MLGiXL85Pfa8uZd6kt11AoYZjhV6ndx7tUFMKrg3Sk4xT-JqpFRKzZe-ZRCbxOZmeF5yN_MNrKtHMi2mb4jSjbH2dsyG4kKc5CsuTh7xJZV91XBDkTbss0G5a2iD_kkpBLsxMAZz8xA5HK7kQBjUJl-fxgJ5-VyNeEuyXCUKbsI3JTNby7p6xyfjXyIBGZRyuCc5Vbr3bAnpChMyTfiSmvQmjLRn3_uUdnJp9FoAwBye9Y2_M6G/https%3A%2F%2Fgithub.com%2Fjokergoo%2FIlluminaHumanMethylationEPICv2anno.20a1.hg38
> Basically they are built with the scripts in the original v1 packages, with
> small adjustment. The scripts for building the two packages are also
> included in the two packages.
> The two v2 packages can be seamlessly integrated into the minfi analysis.
> I also did some simple comparisons between v1 and v2 (
> https://secure-web.cisco.com/1tb2XcerFPyKtnWDHASWp4HiJmKkEZQnpQRX0pCr4zdfYKshBWou5hiaQCMU7fxctqgxTJZHcVXeMDCsdYO4DKDTFGvXu1plvlZqOlJL_XL6wrom9PLgn0bYaqHp3f0gwbDwdR6DndCxxxmy7W8prmtZDI4h4hA_zpjZAQXUS3C77vrKmAB0L2oS_JxbGROozS9GRKkh2dTon2-KX8gl0DDiSGfbubl7xv2mtCcwLhlCW-cQ4W5zpEb7wIk7j1uqvHdAC-jwsG9VPm8VyThNbkBNrijwdrCIfHuggvBKfSknu0oFR42VZ9ifm6JaY3GTm/https%3A%2F%2Fjokergoo.github.io%2FIlluminaHumanMethylationEPICv2manifest%2Farticles%2FIlluminaHumanMethylationEPICv2manifest.html
> ).
> Since the v1 packages were developed by the bioc core members, I want to
> ask is there any plan to support the EPIC v2 array officially? If there is
> no such plan, can I submit the two packages to bioc? If I can, then I will
> have some additional questions regarding how to pass BiocCheck (e.g. file
> size, lazy loading, ...). But that will be in another email.
> Best,
> Zuguang
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