[Bioc-devel] assay dimnames in SingleCellExperiment / SummarizedExperiment
alun at wehi.edu.au
Sat Sep 16 07:43:38 CEST 2017
I'll leave the first point to the SummarizedExperiment maintainers, though I note that your code seems to be about the names of the dimnames rather than the dimnames themselves. (I'm under the impression that consistency in the actual dimnames is enforced somehow by the SE constructor.)
As for the second point; I suppose we could set the second name for the dimnames as "Cells" in SingleCellExperiment, though the choice for the first name is more ambiguous. This request has come up before, and I've never been entirely convinced by its necessity. It seems mostly aesthetic to me, and honestly, if a user doesn't already know that rows are genes and columns are cells, I can't see them flailing away at the keyboard until they call dim() to tell them what the dimensions correspond to.
But I guess other people like aesthetics, so if you want, you can put in a PR to override dim() and dimnames() for SingleCellExperiment to put some names on the returned vectors or lists. If I had to choose, I would go with "Features" and "Cells" for the rows and columns, respectively. (We already use a RSE so we're already implicitly assuming genomic features.)
From: Kevin RUE <kevinrue67 at gmail.com>
Sent: Thursday, 14 September 2017 10:57:39 PM
Cc: davis at ebi.ac.uk; risso.davide at gmail.com; Aaron Lun; Maintainer
Subject: assay dimnames in SingleCellExperiment / SummarizedExperiment
I cc-ed to this email individual package maintainer to directly 'notify' them of this thread and have their respective opinions, but I thought the common use of SummarizedExperiment was worth involving the community as well.
Background: I was updating one of my workflow from SCESet to the SingleCellExperiment class recently introduced on the development branch.
One thing leading to another, I ended up noticing that there is no validity check on dimnames of the various assays in SummarizedExperiment. In other words, the different assays can have different `dimnames` (or some assays can have NULL dimnames). Using the example code from SummarizedExperiment:
nrows <- 200; ncols <- 6
counts3 <- counts2 <- counts <-
matrix(runif(nrows * ncols, 1, 1e4), nrows)
rnames <- paste0("F_", sprintf("%03.f", seq_len(nrows)))
cnames <- LETTERS[1:6]
dimnames(counts) <- list(rnames, cnames)
dimnames(counts2) <- list(Tags = rnames, Samples = cnames)
dimnames(counts3) <- list(Features = rnames, Cells = cnames)
colData <- DataFrame(row.names=cnames)
rse <- SummarizedExperiment(assays=SimpleList(c1=counts, c2=counts2, c3=counts3), colData=colData)
names(dimnames(assay(rse, "c1"))) # NULL
names(dimnames(assay(rse, "c2"))) #  "Tags" "Samples"
names(dimnames(assay(rse, "c3"))) #  "Features" "Cells"
Although not critical, it'd probably be best practice to have a validity check on identical dimnames across all assay, so that one does not have to worry later about `melt` calls returning different column names whether each assay has proper dimnames or not.
The initial glitch that prompted this email related to the `reshape2::melt` method that extracts dimnames, if available, in the `scater::plotHighestExprs` function. Anyway, Davis has already prepared a fix to deal with the scenario whereby the assay does have dimnames (e.g. counts in the edgeR::DGEList class that I generally use to import counts). Somehow that wasn't an issue with the SCESet that I was using previously (probably a side-effect of ExpressionSet).
The point is, the glitch prompted me to think whether a potential standardisation of names(dimnames) could be beneficial, perhaps more specifically in the new `SingleCellExperiment` class (as SummarizedExperiment has a much more general purpose). Considering the fairly specific purpose of the former, I was wondering whether it would be worth:
* enforcing names(dimnames(x)) to "Features" and "Cells", (bearing in mind that features could still be genes, transcripts, ...)
* or maybe dropping dimnames altogether, storing them only once elsewhere (although a slot for that seems overkill)
There may be other possibilities that I haven't thought of yet, but I thought I'd get the ball rolling.
Having well-defined dimnames sounds good practice, with the added benefit of generating aesthetically pleasing column names in melted data-frame as a by-product.
However, I can't tell whether the handling of dimnames is something that needs to be handle by individual downstream package developers, or whether standards should be set in parent classes.
Thanks for your time!
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