[Bioc-devel] rtracklayer::import() fails when genome parameter is used

Martin Morgan martin.morgan at roswellpark.org
Mon Feb 6 17:23:39 CET 2017


On 02/06/2017 10:13 AM, Raymond Cavalcante wrote:
> Oops, I got so caught up in the setting up the code I forgot to say what the error was:
>
> Error in match.arg(pruning.mode) :
>   'arg' should be one of “error”, “coarse”, “fine”, “tidy”

you have a mixture of 'release' (even-numbered y in x.y.z) and 'devel' 
(odd-numbered y in x.y.z) Bioconductor packages, which is a recipe for 
disaster. (Well, disaster might be overstating it a bit...). Use 
BiocInstaller::biocValid() to sort this out (use 
BiocInstaller::biocLite() to manage your packages) and report on whether 
this solves your problem.

Martin

>
> Thanks again,
> Raymond
>
>> On Feb 6, 2017, at 10:10 AM, Raymond Cavalcante <rcavalca at umich.edu> wrote:
>>
>> Hello,
>>
>> Perhaps this is related to problems in GenomeInfoDb::Seqinfo() and NCBI server file structure changes, but rtracklayer::import() is failing when I specify a genome.
>>
>> See below for example code.
>>
>> Thanks,
>> Raymond Cavalcante
>>
>> library(annotatr)
>>
>> # These fail
>> file = system.file('extdata', 'test_BED3.bed', package = 'annotatr')
>> r = annotatr::read_regions(con = file, format = 'bed', genome ='hg19')
>> gr = rtracklayer::import(con = file, format = 'bed', genome='hg19')
>>
>> # These succeed
>> file = system.file('extdata', 'test_BED3.bed', package = 'annotatr')
>> r = annotatr::read_regions(con = file, format = 'bed')
>> gr = rtracklayer::import(con = file, format = 'bed')
>>
>> My devtools::session_info() is:
>>
>> Session info -------------------------------------------------------------------
>> setting  value
>> version  R version 3.3.2 (2016-10-31)
>> system   x86_64, darwin16.1.0
>> ui       unknown
>> language (EN)
>> collate  en_US.UTF-8
>> tz       America/Detroit
>> date     2017-02-06
>>
>> Packages -----------------------------------------------------------------------
>> package                             * version  date
>> AnnotationDbi                         1.36.1   2017-01-27
>> AnnotationHub                         2.6.4    2017-01-27
>> annotatr                            * 1.1.7    2017-01-27
>> assertthat                            0.1      2013-12-06
>> Biobase                               2.34.0   2016-11-16
>> BiocGenerics                          0.20.0   2016-11-16
>> BiocInstaller                         1.24.0   2016-11-16
>> BiocParallel                          1.8.1    2016-11-16
>> biomaRt                               2.30.0   2016-11-16
>> Biostrings                            2.42.1   2017-01-27
>> bitops                                1.0-6    2013-08-17
>> BSgenome                              1.42.0   2016-11-16
>> colorspace                            1.3-1    2016-11-18
>> DBI                                   0.5-1    2016-09-10
>> devtools                              1.12.0   2016-12-05
>> digest                                0.6.10   2016-08-02
>> dplyr                                 0.5.0    2016-06-24
>> GenomeInfoDb                          1.11.7   2017-02-04
>> GenomicAlignments                     1.10.0   2016-11-16
>> GenomicFeatures                       1.26.2   2017-01-27
>> GenomicRanges                         1.26.2   2017-01-27
>> ggplot2                               2.2.1    2016-12-30
>> gtable                                0.2.0    2016-02-26
>> htmltools                             0.3.5    2016-03-21
>> httpuv                                1.3.3    2015-08-04
>> httr                                  1.2.1    2016-07-03
>> interactiveDisplayBase                1.12.0   2016-11-16
>> IRanges                               2.8.1    2017-01-27
>> lattice                               0.20-34  2016-09-06
>> lazyeval                              0.2.0    2016-06-12
>> magrittr                              1.5      2014-11-22
>> Matrix                                1.2-8    2017-01-20
>> memoise                               1.0.0    2016-01-29
>> mime                                  0.5      2016-07-07
>> munsell                               0.4.3    2016-02-13
>> org.Dm.eg.db                          3.4.0    2016-11-16
>> org.Hs.eg.db                          3.4.0    2016-11-16
>> org.Mm.eg.db                          3.4.0    2016-11-16
>> org.Rn.eg.db                          3.4.0    2016-11-16
>> plyr                                  1.8.4    2016-06-08
>> R6                                    2.2.0    2016-10-05
>> Rcpp                                  0.12.9   2017-01-14
>> RCurl                                 1.95-4.8 2016-03-01
>> readr                                 1.0.0    2016-08-03
>> regioneR                              1.6.0    2017-01-27
>> reshape2                              1.4.2    2016-10-22
>> Rsamtools                             1.26.1   2016-11-16
>> RSQLite                               1.1-2    2017-01-08
>> rtracklayer                           1.34.1   2016-11-16
>> S4Vectors                             0.12.1   2017-01-26
>> scales                                0.4.1    2016-11-09
>> shiny                                 0.14.2   2016-11-01
>> stringi                               1.1.2    2016-10-01
>> stringr                               1.1.0    2016-08-19
>> SummarizedExperiment                  1.4.0    2016-11-16
>> tibble                                1.2      2016-08-26
>> TxDb.Dmelanogaster.UCSC.dm3.ensGene   3.2.2    2016-11-16
>> TxDb.Dmelanogaster.UCSC.dm6.ensGene   3.3.0    2016-11-16
>> TxDb.Hsapiens.UCSC.hg19.knownGene     3.2.2    2016-11-16
>> TxDb.Hsapiens.UCSC.hg38.knownGene     3.4.0    2016-11-16
>> TxDb.Mmusculus.UCSC.mm10.knownGene    3.4.0    2016-11-16
>> TxDb.Mmusculus.UCSC.mm9.knownGene     3.2.2    2016-11-16
>> TxDb.Rnorvegicus.UCSC.rn4.ensGene     3.2.2    2016-11-16
>> TxDb.Rnorvegicus.UCSC.rn5.refGene     3.4.0    2016-11-16
>> TxDb.Rnorvegicus.UCSC.rn6.refGene     3.4.0    2016-11-16
>> withr                                 1.0.2    2016-06-20
>> XML                                   3.98-1.5 2016-11-10
>> xtable                                1.8-2    2016-02-05
>> XVector                               0.14.0   2016-11-16
>> yaml                                  2.1.14   2016-11-12
>> zlibbioc                              1.20.0   2016-11-16
>
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