[Bioc-devel] rtracklayer::import() fails when genome parameter is used

Raymond Cavalcante rcavalca at umich.edu
Mon Feb 6 16:10:26 CET 2017


Hello,

Perhaps this is related to problems in GenomeInfoDb::Seqinfo() and NCBI server file structure changes, but rtracklayer::import() is failing when I specify a genome.

See below for example code.

Thanks,
Raymond Cavalcante

library(annotatr)

# These fail
file = system.file('extdata', 'test_BED3.bed', package = 'annotatr')
r = annotatr::read_regions(con = file, format = 'bed', genome ='hg19')
gr = rtracklayer::import(con = file, format = 'bed', genome='hg19')

# These succeed
file = system.file('extdata', 'test_BED3.bed', package = 'annotatr')
r = annotatr::read_regions(con = file, format = 'bed')
gr = rtracklayer::import(con = file, format = 'bed')

My devtools::session_info() is:

Session info -------------------------------------------------------------------
 setting  value                       
 version  R version 3.3.2 (2016-10-31)
 system   x86_64, darwin16.1.0        
 ui       unknown                     
 language (EN)                        
 collate  en_US.UTF-8                 
 tz       America/Detroit             
 date     2017-02-06                  

Packages -----------------------------------------------------------------------
 package                             * version  date      
 AnnotationDbi                         1.36.1   2017-01-27
 AnnotationHub                         2.6.4    2017-01-27
 annotatr                            * 1.1.7    2017-01-27
 assertthat                            0.1      2013-12-06
 Biobase                               2.34.0   2016-11-16
 BiocGenerics                          0.20.0   2016-11-16
 BiocInstaller                         1.24.0   2016-11-16
 BiocParallel                          1.8.1    2016-11-16
 biomaRt                               2.30.0   2016-11-16
 Biostrings                            2.42.1   2017-01-27
 bitops                                1.0-6    2013-08-17
 BSgenome                              1.42.0   2016-11-16
 colorspace                            1.3-1    2016-11-18
 DBI                                   0.5-1    2016-09-10
 devtools                              1.12.0   2016-12-05
 digest                                0.6.10   2016-08-02
 dplyr                                 0.5.0    2016-06-24
 GenomeInfoDb                          1.11.7   2017-02-04
 GenomicAlignments                     1.10.0   2016-11-16
 GenomicFeatures                       1.26.2   2017-01-27
 GenomicRanges                         1.26.2   2017-01-27
 ggplot2                               2.2.1    2016-12-30
 gtable                                0.2.0    2016-02-26
 htmltools                             0.3.5    2016-03-21
 httpuv                                1.3.3    2015-08-04
 httr                                  1.2.1    2016-07-03
 interactiveDisplayBase                1.12.0   2016-11-16
 IRanges                               2.8.1    2017-01-27
 lattice                               0.20-34  2016-09-06
 lazyeval                              0.2.0    2016-06-12
 magrittr                              1.5      2014-11-22
 Matrix                                1.2-8    2017-01-20
 memoise                               1.0.0    2016-01-29
 mime                                  0.5      2016-07-07
 munsell                               0.4.3    2016-02-13
 org.Dm.eg.db                          3.4.0    2016-11-16
 org.Hs.eg.db                          3.4.0    2016-11-16
 org.Mm.eg.db                          3.4.0    2016-11-16
 org.Rn.eg.db                          3.4.0    2016-11-16
 plyr                                  1.8.4    2016-06-08
 R6                                    2.2.0    2016-10-05
 Rcpp                                  0.12.9   2017-01-14
 RCurl                                 1.95-4.8 2016-03-01
 readr                                 1.0.0    2016-08-03
 regioneR                              1.6.0    2017-01-27
 reshape2                              1.4.2    2016-10-22
 Rsamtools                             1.26.1   2016-11-16
 RSQLite                               1.1-2    2017-01-08
 rtracklayer                           1.34.1   2016-11-16
 S4Vectors                             0.12.1   2017-01-26
 scales                                0.4.1    2016-11-09
 shiny                                 0.14.2   2016-11-01
 stringi                               1.1.2    2016-10-01
 stringr                               1.1.0    2016-08-19
 SummarizedExperiment                  1.4.0    2016-11-16
 tibble                                1.2      2016-08-26
 TxDb.Dmelanogaster.UCSC.dm3.ensGene   3.2.2    2016-11-16
 TxDb.Dmelanogaster.UCSC.dm6.ensGene   3.3.0    2016-11-16
 TxDb.Hsapiens.UCSC.hg19.knownGene     3.2.2    2016-11-16
 TxDb.Hsapiens.UCSC.hg38.knownGene     3.4.0    2016-11-16
 TxDb.Mmusculus.UCSC.mm10.knownGene    3.4.0    2016-11-16
 TxDb.Mmusculus.UCSC.mm9.knownGene     3.2.2    2016-11-16
 TxDb.Rnorvegicus.UCSC.rn4.ensGene     3.2.2    2016-11-16
 TxDb.Rnorvegicus.UCSC.rn5.refGene     3.4.0    2016-11-16
 TxDb.Rnorvegicus.UCSC.rn6.refGene     3.4.0    2016-11-16
 withr                                 1.0.2    2016-06-20
 XML                                   3.98-1.5 2016-11-10
 xtable                                1.8-2    2016-02-05
 XVector                               0.14.0   2016-11-16
 yaml                                  2.1.14   2016-11-12
 zlibbioc                              1.20.0   2016-11-16


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