[Bioc-devel] Fwd: Gostats and custom list
amit kumar subudhi
amit4help at gmail.com
Tue Apr 11 14:32:42 CEST 2017
Hi,
I am using Gostats to perform GO enrichment from custom list of genes and
custom annotations. My annotation file looks like this
head (geneGO)
GOterm evi GeneID
1 GO:0016020 ND PCHAS_010020
2 GO:0016021 ND PCHAS_010020
3 GO:0016020 ND PCHAS_010030
4 GO:0016021 ND PCHAS_010030
5 GO:0016020 ND PCHAS_010040
6 GO:0016021 ND PCHAS_010040
My hyperGtest result looks like this
>Over.bp.Phase_0_2 <-hyperGTest(params.bp.Phase_0_2 )
>Over.bp.Phase_0_2
Gene to GO BP Conditional test for over-representation
228 GO BP ids tested (45 have p < 0.05)
Selected gene set size: 37
Gene universe size: 1707
Annotation package: Based on a GeneSetCollection Object
and my summary result looks like this
enrichgobp.Phase_9_12 <-summary(Over.bp.Phase_9_12 )
GOBPID Pvalue OddsRatio ExpCount Count
Size Term V8
1 GO:0019362 4.441045e-05 17.239583 0.43350908 5 20
pyridine nucleotide metabolic process Phase_0_2
2 GO:0046031 1.163932e-04 22.370370 0.28178090 4
13 ADP metabolic process Phase_0_2
3 GO:0006096 1.163932e-04 22.370370 0.28178090 4
13 glycolytic process Phase_0_2
4 GO:0016052 1.163932e-04 22.370370 0.28178090 4
13 carbohydrate catabolic process Phase_0_2
5 GO:0009185 1.604285e-04 20.121212 0.30345636 4 14
ribonucleoside diphosphate metabolic process Phase_0_2
6 GO:0009135 1.604285e-04 20.121212 0.30345636 4 14 purine
nucleoside diphosphate metabolic process Phase_0_2
7 GO:0043436 2.085503e-04 5.450461 2.21089631 9
102 oxoacid metabolic process Phase_0_2
8 GO:0006165 2.153810e-04 18.280992 0.32513181 4 15
nucleoside diphosphate phosphorylation Phase_0_2
9 GO:0051186 1.523968e-03 7.114211 0.88680352 5
42 cofactor metabolic process Phase_0_2
what am I suppose to do, If I want to retrieve the geneIDs associated with
each GOterms. The other information about params is
params.bp.Phase_0_2<-GSEAGOHyperGParams(name="P.chabaudi",geneSetCollection=gsc,geneIds=Phase_0_2
,universeGeneIds=universe,ontology="BP",pvalueCutoff=0.05,conditional=TRUE,
testDirection="over")
Looking forward to hear from you.
With best regards, Amit
--
Amit Kumar Subudhi, PhD
Post Doctoral Research Scientist,
Pathogen Genomics Group,
Division of Biological and Environmental Sciences and Engineering,
Level 4, 4326-WS10, Building 2,
King Abdullah University of Science and Technology (KAUST),
Thuwal 23955-6900, Kingdom of Saudi Arabia,
Phone: (+966 12) 808 0614 <+966%2012%20808%200614>, Mobile: (+966) 5
40375986,
Email Address: amit.subudhi at kaust.edu.sa
--
Amit Kumar Subudhi, PhD
Post Doctoral Research Scientist,
Pathogen Genomics Group,
Division of Biological and Environmental Sciences and Engineering,
Level 4, 4326-WS10, Building 2,
King Abdullah University of Science and Technology (KAUST),
Thuwal 23955-6900, Kingdom of Saudi Arabia,
Phone: (+966 12) 808 0614, Mobile: (+966) 5 40375986,
Email Address: amit.subudhi at kaust.edu.sa
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