[Bioc-devel] Debugging build error on Linux machine: is it using rtracklayer >= 1.33.11?

Dan Tenenbaum dtenenba at fredhutch.org
Tue Aug 9 21:30:26 CEST 2016


Actually it looks like it was a permissions issue with the directory /tmp/udcCache. I removed this directory (as superuser) and that error no longer happens.

Now I get a different error which seems unrelated:

...
* creating vignettes ... ERROR
Quitting from lines 2-32 (SRP009615-results.Rmd)
Error: processing vignette 'SRP009615-results.Rmd' failed with diagnostics:
object 'software' not found
Execution halted


Probably because of line 20:

Basic `r software` results exploration

`r software` is interpreted as a chunk of code to be executed by R.


Dan


----- Original Message -----
> From: "Dan Tenenbaum" <dtenenba at fredhutch.org>
> To: "lcollado" <lcollado at jhu.edu>
> Cc: "Jeff Leek" <jtleek at gmail.com>, "bioc-devel" <bioc-devel at r-project.org>, "Andrew Jaffe" <andrewejaffe at gmail.com>,
> "Ben Langmead" <langmea at cs.jhu.edu>, "Abhi Nellore" <anellore at gmail.com>
> Sent: Tuesday, August 9, 2016 11:46:32 AM
> Subject: Re: [Bioc-devel] Debugging build error on Linux machine: is it using	rtracklayer >= 1.33.11?

> 1.33.11 is the latest version in svn.
> 
> https://www.bioconductor.org/checkResults/3.4/bioc-LATEST/zin1-R-instpkgs.html
> will tell you (with a reasonable degree of accuracy) which version of which
> package is installed on zin1, it reports that it has version 1.33.11 installed.
> 
> The issue with recount looks like it's a (hopefully transient) UCSC connection
> problem.
> 
> Dan
> 
> 
> ----- Original Message -----
>> From: "lcollado" <lcollado at jhu.edu>
>> To: "bioc-devel" <bioc-devel at r-project.org>
>> Cc: "Jeff Leek" <jtleek at gmail.com>, "Andrew Jaffe" <andrewejaffe at gmail.com>,
>> "Ben Langmead" <langmea at cs.jhu.edu>, "Abhi
>> Nellore" <anellore at gmail.com>
>> Sent: Tuesday, August 9, 2016 11:33:50 AM
>> Subject: [Bioc-devel] Debugging build error on Linux machine: is it using
>> 	rtracklayer >= 1.33.11?
> 
>> Hi,
>> 
>> Currently the build for `recount` is failing in the Linux build
>> machine
>> http://www.bioconductor.org/checkResults/3.4/bioc-LATEST/recount/zin1-buildsrc.html.
>> It's been failing with the same error message for a while. The build
>> message ends with:
>> 
>> Setting options('download.file.method.GEOquery'='auto')
>> Setting options('GEOquery.inmemory.gpl'=FALSE)
>> trying URL 'http://duffel.rail.bio/recount/SRP009615/rse_gene.Rdata'
>> Content type 'application/octet-stream' length 1383643 bytes (1.3 MB)
>> ==================================================
>> downloaded 1.3 MB
>> 
>> Quitting from lines 400-406 (recount-quickstart.Rmd)
>> Error: processing vignette 'recount-quickstart.Rmd' failed with diagnostics:
>> UCSC library operation failed
>> Execution halted
>> 
>> 
>> The lines of code from the recount vignette this refers to are:
>> 
>> ## Define expressed regions for study SRP009615, only for chromosome Y
>> regions <- expressed_regions('SRP009615', 'chrY', cutoff = 5L,
>>    maxClusterGap = 3000L)
>> 
>> ## Briefly explore the resulting regions
>> regions
>> 
>> 
>> 
>> The UCSC error is something I've seen in previous versions of
>> rtracklayer, which have been addressed in rtracklayer 1.33.11. recount
>> version 0.99.25 (which is the one from the build report) had under
>> suggests rtracklayer (>= 1.33.11). I thought that this would mean that
>> the build would fail if rtracklayer below version 1.33.11 was being
>> used. I'm no longer sure, which is why in recount version 0.99.27 I
>> moved rtracklayer to imports to test this. The latest build report
>> shows that rtracklayer 1.33.11 did build correctly in the Linux
>> machine.
>> 
>> 
>> Everything seems to be working fine in my local tests and in R Travis
>> https://travis-ci.org/leekgroup/recount/builds/150995800. Below is
>> some code showing that everything is working as expected in my local
>> Linux test. So I'm a tad confused and can't reproduce this issue when
>> using rtracklayer version 1.33.11. Which is why I'm guessing that
>> somehow the Linux build machine is not using rtracklayer >= 1.33.11.
>> 
>> 
>> 
>> 
>> ## The url redirects as expected to the correct url
>>> rtracklayer:::expandURL('http://duffel.rail.bio/recount/SRP009615/rse_gene.Rdata')
>> [1] "http://idies.jhu.edu/recount/data/SRP009615/rse_gene.Rdata"
>>> library('recount')
>> Loading required package: SummarizedExperiment
>> Loading required package: GenomicRanges
>> Loading required package: stats4
>> Loading required package: BiocGenerics
>> Loading required package: parallel
>> 
>> Attaching package: ‘BiocGenerics’
>> 
>> The following objects are masked from ‘package:parallel’:
>> 
>>    clusterApply, clusterApplyLB, clusterCall, clusterEvalQ,
>>    clusterExport, clusterMap, parApply, parCapply, parLapply,
>>    parLapplyLB, parRapply, parSapply, parSapplyLB
>> 
>> The following objects are masked from ‘package:stats’:
>> 
>>    IQR, mad, xtabs
>> 
>> The following objects are masked from ‘package:base’:
>> 
>>    anyDuplicated, append, as.data.frame, cbind, colnames, do.call,
>>    duplicated, eval, evalq, Filter, Find, get, grep, grepl, intersect,
>>    is.unsorted, lapply, lengths, Map, mapply, match, mget, order,
>>    paste, pmax, pmax.int, pmin, pmin.int, Position, rank, rbind,
>>    Reduce, rownames, sapply, setdiff, sort, table, tapply, union,
>>    unique, unsplit, which, which.max, which.min
>> 
>> Loading required package: S4Vectors
>> 
>> Attaching package: ‘S4Vectors’
>> 
>> The following objects are masked from ‘package:base’:
>> 
>>    colMeans, colSums, expand.grid, rowMeans, rowSums
>> 
>> Loading required package: IRanges
>> Loading required package: GenomeInfoDb
>> Loading required package: Biobase
>> Welcome to Bioconductor
>> 
>>    Vignettes contain introductory material; view with
>>    'browseVignettes()'. To cite Bioconductor, see
>>    'citation("Biobase")', and for packages 'citation("pkgname")'.
>> 
>> Setting options('download.file.method.GEOquery'='auto')
>> Setting options('GEOquery.inmemory.gpl'=FALSE)
>>>
>>> ## Define expressed regions for study SRP009615, only for chromosome Y
>>> regions <- expressed_regions('SRP009615', 'chrY', cutoff = 5L,
>> +     maxClusterGap = 3000L)
>> 2016-08-09 14:16:59 loadCoverage: loading BigWig file
>> http://duffel.rail.bio/recount/SRP009615/bw/mean_SRP009615.bw
>> 2016-08-09 14:17:01 loadCoverage: applying the cutoff to the merged data
>> 2016-08-09 14:17:02 filterData: originally there were 57227415 rows,
>> now there are 57227415 rows. Meaning that 0 percent was filtered.
>> 2016-08-09 14:17:02 findRegions: identifying potential segments
>> 2016-08-09 14:17:02 findRegions: segmenting information
>> 2016-08-09 14:17:02 .getSegmentsRle: segmenting with cutoff(s) 5
>> 2016-08-09 14:17:02 findRegions: identifying candidate regions
>> 2016-08-09 14:17:03 findRegions: identifying region clusters
>>>
>>> ## Briefly explore the resulting regions
>>> regions
>> GRanges object with 808 ranges and 6 metadata columns:
>>      seqnames               ranges strand |            value             area
>>         <Rle>            <IRanges>  <Rle> |        <numeric>        <numeric>
>>    1     chrY   [2929794, 2929829]      * | 14.7265009482702 530.154034137726
>>    2     chrY   [2956678, 2956701]      * | 12.8106340567271  307.45521736145
>>    3     chrY   [2977203, 2977227]      * | 5.34908433914185 133.727108478546
>>    4     chrY   [2977957, 2977994]      * | 6.46976616508082 245.851114273071
>>    5     chrY   [2978850, 2978871]      * |  5.7976552139629 127.548414707184
>>  ...      ...                  ...    ... .              ...              ...
>>  804     chrY [26614511, 26614546]      * | 7.28189378314548 262.148176193237
>>  805     chrY [26614548, 26614553]      * | 5.48768202463786 32.9260921478271
>>  806     chrY [26614779, 26614808]      * | 6.64339276949565 199.301783084869
>>  807     chrY [26626808, 26626848]      * | 12.6038152648181 516.756425857544
>>  808     chrY [26626971, 26627028]      * | 14.1673366941255 821.705528259277
>>      indexStart  indexEnd cluster clusterL
>>       <integer> <integer>   <Rle>    <Rle>
>>    1    2929794   2929829       1       36
>>    2    2956678   2956701       2       24
>>    3    2977203   2977227       3     2750
>>    4    2977957   2977994       3     2750
>>    5    2978850   2978871       3     2750
>>  ...        ...       ...     ...      ...
>>  804   26614511  26614546     224      298
>>  805   26614548  26614553     224      298
>>  806   26614779  26614808     224      298
>>  807   26626808  26626848     225      221
>>  808   26626971  26627028     225      221
>>  -------
>>  seqinfo: 1 sequence from an unspecified genome
>>>
>>> options(width = 120)
>>> devtools::session_info()
>> Session info
>> -----------------------------------------------------------------------------------------------------------
>> setting  value
>> version  R version 3.3.1 Patched (2016-07-11 r70891)
>> system   x86_64, linux-gnu
>> ui       X11
>> language (EN)
>> collate  en_US.UTF-8
>> tz       <NA>
>> date     2016-08-09
>> 
>> Packages
>> ---------------------------------------------------------------------------------------------------------------
>> package              * version  date       source
>> acepack                1.3-3.3  2014-11-24 CRAN (R 3.3.0)
>> AnnotationDbi          1.35.4   2016-07-22 Bioconductor
>> Biobase              * 2.33.0   2016-05-08 Bioconductor
>> BiocGenerics         * 0.19.2   2016-07-22 Bioconductor
>> BiocParallel           1.7.6    2016-08-09 cran (@1.7.6)
>> biomaRt                2.29.2   2016-06-07 Bioconductor
>> Biostrings             2.41.4   2016-07-22 Bioconductor
>> bitops                 1.0-6    2013-08-17 CRAN (R 3.3.0)
>> BSgenome               1.41.2   2016-07-22 Bioconductor
>> bumphunter             1.13.1   2016-07-22 Bioconductor
>> chron                  2.3-47   2015-06-24 CRAN (R 3.3.0)
>> cluster                2.0.4    2016-04-18 CRAN (R 3.3.1)
>> codetools              0.2-14   2015-07-15 CRAN (R 3.3.1)
>> colorspace             1.2-6    2015-03-11 CRAN (R 3.3.0)
>> data.table             1.9.6    2015-09-19 CRAN (R 3.3.1)
>> DBI                    0.4-1    2016-05-08 CRAN (R 3.3.0)
>> derfinder              1.7.9    2016-07-22 Bioconductor
>> derfinderHelper        1.7.3    2016-07-22 Bioconductor
>> devtools               1.12.0   2016-06-24 CRAN (R 3.3.1)
>> digest                 0.6.9    2016-01-08 CRAN (R 3.3.0)
>> doRNG                  1.6      2014-03-07 CRAN (R 3.3.0)
>> downloader             0.4      2015-07-09 cran (@0.4)
>> foreach                1.4.3    2015-10-13 CRAN (R 3.3.0)
>> foreign                0.8-66   2015-08-19 CRAN (R 3.3.1)
>> Formula                1.2-1    2015-04-07 CRAN (R 3.3.0)
>> GenomeInfoDb         * 1.9.4    2016-07-22 Bioconductor
>> GenomicAlignments      1.9.6    2016-07-22 Bioconductor
>> GenomicFeatures        1.25.15  2016-07-22 Bioconductor
>> GenomicFiles           1.9.11   2016-07-22 Bioconductor
>> GenomicRanges        * 1.25.93  2016-08-09 cran (@1.25.93)
>> GEOquery               2.39.3   2016-06-15 Bioconductor
>> ggplot2                2.1.0    2016-03-01 CRAN (R 3.3.1)
>> gridExtra              2.2.1    2016-02-29 CRAN (R 3.3.0)
>> gtable                 0.2.0    2016-02-26 CRAN (R 3.3.0)
>> Hmisc                  3.17-4   2016-05-02 CRAN (R 3.3.1)
>> httr                   1.2.1    2016-07-03 cran (@1.2.1)
>> IRanges              * 2.7.12   2016-08-09 cran (@2.7.12)
>> iterators              1.0.8    2015-10-13 CRAN (R 3.3.0)
>> jsonlite               1.0      2016-07-01 CRAN (R 3.3.1)
>> lattice                0.20-33  2015-07-14 CRAN (R 3.3.1)
>> latticeExtra           0.6-28   2016-02-09 CRAN (R 3.3.0)
>> locfit                 1.5-9.1  2013-04-20 CRAN (R 3.3.0)
>> magrittr               1.5      2014-11-22 CRAN (R 3.3.1)
>> Matrix                 1.2-6    2016-05-02 CRAN (R 3.3.1)
>> matrixStats            0.50.2   2016-04-24 CRAN (R 3.3.1)
>> memoise                1.0.0    2016-01-29 CRAN (R 3.3.0)
>> munsell                0.4.3    2016-02-13 CRAN (R 3.3.0)
>> nnet                   7.3-12   2016-02-02 CRAN (R 3.3.1)
>> pkgmaker               0.22     2014-05-14 CRAN (R 3.3.0)
>> plyr                   1.8.4    2016-06-08 CRAN (R 3.3.1)
>> qvalue                 2.5.2    2016-07-22 Bioconductor
>> R6                     2.1.2    2016-01-26 CRAN (R 3.3.0)
>> RColorBrewer           1.1-2    2014-12-07 CRAN (R 3.3.0)
>> Rcpp                   0.12.6   2016-07-19 CRAN (R 3.3.1)
>> RCurl                  1.95-4.8 2016-03-01 CRAN (R 3.3.0)
>> recount              * 0.99.27  2016-08-09 Github (leekgroup/recount at 922e7a6)
>> registry               0.3      2015-07-08 CRAN (R 3.3.0)
>> rentrez                1.0.2    2016-04-21 cran (@1.0.2)
>> reshape2               1.4.1    2014-12-06 CRAN (R 3.3.0)
>> rngtools               1.2.4    2014-03-06 CRAN (R 3.3.0)
>> rpart                  4.1-10   2015-06-29 CRAN (R 3.3.1)
>> Rsamtools              1.25.0   2016-07-22 Bioconductor
>> RSQLite                1.0.0    2014-10-25 CRAN (R 3.3.0)
>> rtracklayer            1.33.11  2016-07-22 Github
>> (Bioconductor-mirror/rtracklayer at c12bd86)
>> S4Vectors            * 0.11.10  2016-07-22 Bioconductor
>> scales                 0.4.0    2016-02-26 CRAN (R 3.3.0)
>> stringi                1.1.1    2016-05-27 CRAN (R 3.3.0)
>> stringr                1.0.0    2015-04-30 CRAN (R 3.3.1)
>> SummarizedExperiment * 1.3.81   2016-08-09 cran (@1.3.81)
>> survival               2.39-5   2016-06-26 CRAN (R 3.3.1)
>> VariantAnnotation      1.19.8   2016-07-22 Bioconductor
>> withr                  1.0.2    2016-06-20 CRAN (R 3.3.1)
>> XML                    3.98-1.4 2016-03-01 CRAN (R 3.3.0)
>> xtable                 1.8-2    2016-02-05 CRAN (R 3.3.0)
>> XVector                0.13.7   2016-08-09 cran (@0.13.7)
>> zlibbioc               1.19.0   2016-05-08 Bioconductor
>>>
>> 
>> Note that in my local test, I installed rtracklayer from the
>> Bioconductor-mirror. In my Travis test, rtracklayer gets installed
>> using biocLite().
>> 
>> 
>> I know that the latest Windows build failed, but that's because of the
>> recurrent zlibbioc issue. I've seen it build and pass R CMD check
>> before in moscato1.
>> 
>> 
>> I let this issue linger because we wanted to post our pre-print to
>> bioRxiv before @bioconductor automatically tweeted about the recount
>> package. However, now that the pre-print is up, we'd like to have the
>> recount page http://bioconductor.org/packages/recount/ built (which I
>> know depends on the Linux build working).
>> 
>> Best,
>> Leo
>> 
>> _______________________________________________
>> Bioc-devel at r-project.org mailing list
>> https://stat.ethz.ch/mailman/listinfo/bioc-devel
> 
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