[Bioc-devel] chromosome lengths (seqinfo) for supported BSgenome builds into GenomeInfoDb?

Tim Triche, Jr. tim.triche at gmail.com
Fri Jun 5 22:39:24 CEST 2015


how about just

gr <- addSeqinfo(gr, "hg19")

Statistics is the grammar of science.
Karl Pearson <http://en.wikipedia.org/wiki/The_Grammar_of_Science>

On Fri, Jun 5, 2015 at 1:36 PM, Hervé Pagès <hpages at fredhutch.org> wrote:

> On 06/05/2015 01:19 PM, Michael Lawrence wrote:
>
>> To support the multi-genome case, one could set the genome as a
>> vector, one value for each seqname, and it would fix the
>> style/seqlength per seqname. It could sort by the combination of
>> seqname and species. Presumably it would do nothing for unknown
>> genomes.
>>
>> But I agree that a standardizeSeqinfo() that amounts to "genome(x) <-
>> genome(x)" would make sense.
>>
>> I don't think people sort too often by seqnames (except to the
>> "natural" ordering),
>>
>
> That's what sort(), order(), rank() do by default: they sort by seqnames
> first, then by start, then by end, and finally by strand.
>
>  but I could be wrong. I do sympathize though with
>> the need for a low-level accessor. At least one would want a parameter
>> for disabling the standardization.
>>
>
> Ok. So the candidates are:
>
>  (a) standardizeSeqinfo(gr) <- "hg19"
>
>  (b) gr <- standardizeSeqinfo(gr, "hg19")
>
>  (c) standardizeGenome(gr) <- "hg19"
>
>  (d) gr <- standardizeGenome(gr, "hg19")
>
>  (e) seqinfo(gr) <- "hg19"
>
> Is there a risk of confusion with keepStandardChromosomes where
> "standard" means a very different thing? I'll add 2 more:
>
>  (f) normalizeSeqinfo(gr) <- "hg19"
>
>  (g) gr <- normalizeSeqinfo(gr, "hg19")
>
> Anyway, we're not here yet. As pointed in an earlier post, there are
> still some missing pieces to complete the puzzle.
>
> Thanks,
> H.
>
>
>> On Fri, Jun 5, 2015 at 12:54 PM, Kasper Daniel Hansen
>> <kasperdanielhansen at gmail.com> wrote:
>>
>>> In WGBS we frequently sequence a human with spikein from the lambda
>>> genome.
>>> In this case, most of the chromosomes of the Granges are from human,
>>> except
>>> one.  This is a usecase where genome(GR) is not constant.  I suggest,
>>> partly
>>> for compatibility, to keep genome, but perhaps do something like
>>>    standardizeGenome()
>>> or something like this.
>>>
>>> I would indeed love, love, love a function which just cleans it up.
>>>
>>> Kasper
>>>
>>> On Fri, Jun 5, 2015 at 2:51 PM, Gabe Becker <becker.gabe at gene.com>
>>> wrote:
>>>
>>>>
>>>> Herve,
>>>>
>>>> This is probably a naive question, but what usecases are there for
>>>> creating
>>>> an object with the wrong seqinfo for its genome?
>>>>
>>>> ~G
>>>>
>>>> On Fri, Jun 5, 2015 at 11:43 AM, Michael Lawrence
>>>> <lawrence.michael at gene.com
>>>>
>>>>> wrote:
>>>>>
>>>>
>>>>  On Thu, Jun 4, 2015 at 11:48 PM, Hervé Pagès <hpages at fredhutch.org>
>>>>> wrote:
>>>>>
>>>>>> I also think that we're heading towards something like that.
>>>>>>
>>>>>> So genome(gr) <- "hg19" would:
>>>>>>
>>>>>>    (a) Add any missing information to the seqinfo.
>>>>>>    (b) Sort the seqlevels in "canonical" order.
>>>>>>    (c) Change the seqlevels style to UCSC style if they are not.
>>>>>>
>>>>>> The 3 tasks are orthogonal. I guess most of the times people want
>>>>>> an easy way to perform them all at once.
>>>>>>
>>>>>> We could easily support (a) and (b). This assumes that the current
>>>>>> seqlevels are already valid hg19 seqlevels:
>>>>>>
>>>>>>    si1 <- Seqinfo(c("chrX", "chrUn_gl000249", "chr2",
>>>>>> "chr6_cox_hap2"))
>>>>>>    gr1 <- GRanges(seqinfo=si1)
>>>>>>    hg19_si <- Seqinfo(genome="hg19")
>>>>>>
>>>>>>    ## (a):
>>>>>>    seqinfo(gr1) <- merge(seqinfo(gr1), hg19_si)[seqlevels(gr1)]
>>>>>>    seqinfo(gr1)
>>>>>>    # Seqinfo object with 4 sequences (1 circular) from hg19 genome:
>>>>>>    #   seqnames       seqlengths isCircular genome
>>>>>>    #   chrX            155270560      FALSE   hg19
>>>>>>    #   chrUn_gl000249      38502      FALSE   hg19
>>>>>>    #   chr2            243199373      FALSE   hg19
>>>>>>    #   chr6_cox_hap2     4795371      FALSE   hg19
>>>>>>
>>>>>>    ## (b):
>>>>>>    seqlevels(gr1) <- intersect(seqlevels(hg19_si), seqlevels(gr1))
>>>>>>    seqinfo(gr1)
>>>>>>    # Seqinfo object with 4 sequences (1 circular) from hg19 genome:
>>>>>>    #   seqnames       seqlengths isCircular genome
>>>>>>    #   chr2            243199373      FALSE   hg19
>>>>>>    #   chrX            155270560      FALSE   hg19
>>>>>>    #   chr6_cox_hap2     4795371      FALSE   hg19
>>>>>>    #   chrUn_gl000249      38502      FALSE   hg19
>>>>>>
>>>>>> (c) is harder because seqlevelsStyle() doesn't know how to rename
>>>>>> scaffolds yet:
>>>>>>
>>>>>>    si2 <- Seqinfo(c("X", "HSCHRUN_RANDOM_CTG42", "2",
>>>>>>
>>>>> "HSCHR6_MHC_COX_CTG1"))
>>>>>
>>>>>>    gr2 <- GRanges(seqinfo=si2)
>>>>>>
>>>>>>    seqlevelsStyle(gr2)
>>>>>>    # [1] "NCBI"
>>>>>>
>>>>>>    seqlevelsStyle(gr2) <- "UCSC"
>>>>>>    seqlevels(gr2)
>>>>>>    # [1] "chrX"                 "HSCHRUN_RANDOM_CTG42" "chr2"
>>>>>>    # [4] "HSCHR6_MHC_COX_CTG1"
>>>>>>
>>>>>> So we need to work on this.
>>>>>>
>>>>>> I'm not sure about using genome(gr) <- "hg19" for this. Right now
>>>>>> it sets the genome column of the seqinfo with the supplied string
>>>>>> and nothing else. Aren't there valid use cases for this?
>>>>>>
>>>>>
>>>>> Not sure. People would almost always want the seqname style and order
>>>>> to be consistent with the given genome.
>>>>>
>>>>>  What about
>>>>>> using seqinfo(gr) <- "hg19" instead? It kind of suggests that the
>>>>>> whole seqinfo component actually gets filled.
>>>>>>
>>>>>>
>>>>> Yea, but "genome" is so intuitive compared to "seqinfo".
>>>>>
>>>>>
>>>>>
>>>>>  H.
>>>>>>
>>>>>> On 06/04/2015 06:30 PM, Tim Triche, Jr. wrote:
>>>>>>
>>>>>>>
>>>>>>> that's kind of always been my goal...
>>>>>>>
>>>>>>>
>>>>>>> Statistics is the grammar of science.
>>>>>>> Karl Pearson <http://en.wikipedia.org/wiki/The_Grammar_of_Science>
>>>>>>>
>>>>>>> On Thu, Jun 4, 2015 at 6:29 PM, Michael Lawrence
>>>>>>> <lawrence.michael at gene.com <mailto:lawrence.michael at gene.com>>
>>>>>>> wrote:
>>>>>>>
>>>>>>>      Maybe this could eventually support setting the seqinfo with:
>>>>>>>
>>>>>>>      genome(gr) <- "hg19"
>>>>>>>
>>>>>>>      Or is that being too clever?
>>>>>>>
>>>>>>>      On Thu, Jun 4, 2015 at 4:28 PM, Hervé Pagès <
>>>>>>> hpages at fredhutch.org
>>>>>>>      <mailto:hpages at fredhutch.org>> wrote:
>>>>>>>       > Hi,
>>>>>>>       >
>>>>>>>       > FWIW I started to work on supporting quick generation of a
>>>>>>> standalone
>>>>>>>       > Seqinfo object via Seqinfo(genome="hg38") in GenomeInfoDb.
>>>>>>>       >
>>>>>>>       > It already supports hg38, hg19, hg18, panTro4, panTro3,
>>>>>>> panTro2,
>>>>>>>       > bosTau8, bosTau7, bosTau6, canFam3, canFam2, canFam1,
>>>>>>> musFur1,
>>>>>>> mm10,
>>>>>>>       > mm9, mm8, susScr3, susScr2, rn6, rheMac3, rheMac2, galGal4,
>>>>>>> galGal3,
>>>>>>>       > gasAcu1, danRer7, apiMel2, dm6, dm3, ce10, ce6, ce4, ce2,
>>>>>>>
>>>>>> sacCer3,
>>>>>
>>>>>>       > and sacCer2. I'll add more.
>>>>>>>       >
>>>>>>>       > See ?Seqinfo for some examples.
>>>>>>>       >
>>>>>>>       > Right now it fetches the information from internet every time
>>>>>>> you
>>>>>>>       > call it but maybe we should just store that information in
>>>>>>> the
>>>>>>>       > GenomeInfoDb package as Tim suggested?
>>>>>>>       >
>>>>>>>       > H.
>>>>>>>       >
>>>>>>>       >
>>>>>>>       > On 06/03/2015 12:54 PM, Tim Triche, Jr. wrote:
>>>>>>>       >>
>>>>>>>       >> That would be perfect actually.  And it would radically
>>>>>>> reduce &
>>>>>>>       >> modularize maintenance.  Maybe that's the best way to go
>>>>>>> after
>>>>>>>      all.  Quite
>>>>>>>       >> sensible.
>>>>>>>       >>
>>>>>>>       >> --t
>>>>>>>       >>
>>>>>>>       >>> On Jun 3, 2015, at 12:46 PM, Vincent Carey
>>>>>>>      <stvjc at channing.harvard.edu <mailto:stvjc at channing.harvard.edu
>>>>>>> >>
>>>>>>>       >>> wrote:
>>>>>>>       >>>
>>>>>>>       >>> It really isn't hard to have multiple OrganismDb packages
>>>>>>> in
>>>>>>>      place -- the
>>>>>>>       >>> process of making new ones is documented and was given as
>>>>>>> an
>>>>>>>      exercise in
>>>>>>>       >>> the EdX course.  I don't know if we want to
>>>>>>> institutionalize
>>>>>>> it
>>>>>>> and
>>>>>>>       >>> distribute such -- I think we might, so that there would be
>>>>>>>      Hs19, Hs38,
>>>>>>>       >>> mm9, etc. packages.  They have very little content, they
>>>>>>> just
>>>>>>>      coordinate
>>>>>>>       >>> interactions with packages that you'll already have.
>>>>>>>       >>>
>>>>>>>       >>> On Wed, Jun 3, 2015 at 3:26 PM, Tim Triche, Jr.
>>>>>>>      <tim.triche at gmail.com <mailto:tim.triche at gmail.com>>
>>>>>>>
>>>>>>>       >>> wrote:
>>>>>>>       >>>
>>>>>>>       >>>> Right, I typically do that too, and if you're working on
>>>>>>> human
>>>>>>>      data it
>>>>>>>       >>>> isn't a big deal.  What makes things a lot more of a drag
>>>>>>> is
>>>>>>>      when you
>>>>>>>       >>>> work
>>>>>>>       >>>> on e.g. mouse data (mm9 vs mm10, aka GRCm37 vs GRCm38)
>>>>>>> where
>>>>>>>       >>>> Mus.musculus
>>>>>>>       >>>> is essentially a "build ahead" of Homo.sapiens.
>>>>>>>       >>>>
>>>>>>>       >>>> R> seqinfo(Homo.sapiens)
>>>>>>>       >>>> Seqinfo object with 93 sequences (1 circular) from hg19
>>>>>>> genome
>>>>>>>       >>>>
>>>>>>>       >>>> R> seqinfo(Mus.musculus)
>>>>>>>       >>>> Seqinfo object with 66 sequences (1 circular) from mm10
>>>>>>>
>>>>>> genome:
>>>>>
>>>>>>       >>>>
>>>>>>>       >>>> It's not as explicit as directly assigning the seqinfo
>>>>>>> from
>>>>>>> a
>>>>>>>      genome
>>>>>>>       >>>> that
>>>>>>>       >>>> corresponds to that of your annotations/results/whatever.
>>>>>>> I
>>>>>>>      know we
>>>>>>>       >>>> could
>>>>>>>       >>>> all use crossmap or liftOver or whatever, but that's not
>>>>>>>      really the
>>>>>>>       >>>> same,
>>>>>>>       >>>> and it takes time, whereas assigning the proper seqinfo
>>>>>>> for
>>>>>>>       >>>> relationships
>>>>>>>       >>>> is very fast.
>>>>>>>       >>>>
>>>>>>>       >>>> That's all I was getting at...
>>>>>>>       >>>>
>>>>>>>       >>>>
>>>>>>>       >>>> Statistics is the grammar of science.
>>>>>>>       >>>> Karl Pearson
>>>>>>> <http://en.wikipedia.org/wiki/The_Grammar_of_Science>
>>>>>>>       >>>>
>>>>>>>       >>>> On Wed, Jun 3, 2015 at 12:17 PM, Vincent Carey
>>>>>>>       >>>> <stvjc at channing.harvard.edu <mailto:
>>>>>>>
>>>>>> stvjc at channing.harvard.edu>
>>>>>
>>>>>>       >>>>>
>>>>>>>       >>>>> wrote:
>>>>>>>       >>>>
>>>>>>>       >>>>
>>>>>>>       >>>>> I typically get this info from Homo.sapiens.  The result
>>>>>>> is
>>>>>>>      parasitic
>>>>>>>       >>>>> on
>>>>>>>       >>>>> the TxDb that is in there.  I don't know how easy it is
>>>>>>> to
>>>>>>>
>>>>>> swap
>>>>>
>>>>>>       >>>>> alternate
>>>>>>>       >>>>> TxDb in to get a different build.  I think it would make
>>>>>>>
>>>>>> sense
>>>>>
>>>>>> to
>>>>>>>       >>>>> regard
>>>>>>>       >>>>> the OrganismDb instances as foundational for this sort of
>>>>>>>      structural
>>>>>>>       >>>>> data.
>>>>>>>       >>>>>
>>>>>>>       >>>>> On Wed, Jun 3, 2015 at 3:12 PM, Kasper Daniel Hansen <
>>>>>>>       >>>>> kasperdanielhansen at gmail.com
>>>>>>>      <mailto:kasperdanielhansen at gmail.com>> wrote:
>>>>>>>       >>>>>
>>>>>>>       >>>>>> Let me rephrase this slightly.  From one POV the purpose
>>>>>>> of
>>>>>>>       >>>>>> GenomeInfoDb
>>>>>>>       >>>>>> is
>>>>>>>       >>>>>> clean up the seqinfo slot.  Currently it does most of
>>>>>>> the
>>>>>>>      cleaning,
>>>>>>>       >>>>>> but
>>>>>>>       >>>>>> it
>>>>>>>       >>>>>> does not add seqlengths.
>>>>>>>       >>>>>>
>>>>>>>       >>>>>> It is clear that seqlengths depends on the version of
>>>>>>> the
>>>>>>>      genome, but
>>>>>>>       >>>>>> I
>>>>>>>       >>>>>> will argue so does the seqnames.  Of course, for human,
>>>>>>>      chr22 will not
>>>>>>>       >>>>>> change.  But what about the names of all the random
>>>>>>>      contigs?  Or for
>>>>>>>       >>>>>> other
>>>>>>>       >>>>>> organisms, what about going from a draft genome with 10k
>>>>>>>      contigs to a
>>>>>>>       >>>>>> more
>>>>>>>       >>>>>> completely genome assembled into fewer, larger
>>>>>>> chromosomes.
>>>>>>>       >>>>>>
>>>>>>>       >>>>>> I acknowledge that this information is present in the
>>>>>>>
>>>>>> BSgenome
>>>>>
>>>>>>       >>>>>> packages,
>>>>>>>       >>>>>> but it seems (to me) to be very appropriate to have them
>>>>>>>      around for
>>>>>>>       >>>>>> cleaning up the seqinfo slot.  For some situations it is
>>>>>>> not
>>>>>>>      great to
>>>>>>>       >>>>>> depend on 1 GB> download for something that is a few
>>>>>>> bytes.
>>>>>>>       >>>>>>
>>>>>>>       >>>>>> Best,
>>>>>>>       >>>>>> Kasper
>>>>>>>       >>>>>>
>>>>>>>       >>>>>> On Wed, Jun 3, 2015 at 3:00 PM, Tim Triche, Jr.
>>>>>>>      <tim.triche at gmail.com <mailto:tim.triche at gmail.com>>
>>>>>>>
>>>>>>>       >>>>>> wrote:
>>>>>>>       >>>>>>
>>>>>>>       >>>>>>> It would be nice (for a number of reasons) to have
>>>>>>>      chromosome lengths
>>>>>>>       >>>>>>> readily available in a foundational package like
>>>>>>>      GenomeInfoDb, so
>>>>>>>       >>>>>>> that,
>>>>>>>       >>>>>>> say,
>>>>>>>       >>>>>>>
>>>>>>>       >>>>>>> data(seqinfo.hg19)
>>>>>>>       >>>>>>> seqinfo(myResults) <- seqinfo.hg19[
>>>>>>> seqlevels(myResults)
>>>>>>> ]
>>>>>>>       >>>>>>>
>>>>>>>       >>>>>>> would work without issues.  Is there any particular
>>>>>>> reason
>>>>>>> this
>>>>>>>       >>>>>>
>>>>>>>       >>>>>> couldn't
>>>>>>>       >>>>>>>
>>>>>>>       >>>>>>> happen for the supported/available BSgenomes?  It would
>>>>>>>      seem like a
>>>>>>>       >>>>>>
>>>>>>>       >>>>>> simple
>>>>>>>       >>>>>>>
>>>>>>>       >>>>>>> matter to do
>>>>>>>       >>>>>>>
>>>>>>>       >>>>>>> R> library(BSgenome.Hsapiens.UCSC.hg19)
>>>>>>>       >>>>>>> R> seqinfo.hg19 <- seqinfo(Hsapiens)
>>>>>>>       >>>>>>> R> save(seqinfo.hg19,
>>>>>>>       >>>>>>> file="~/bioc-devel/GenomeInfoDb/data/seqinfo.hg19.rda")
>>>>>>>       >>>>>>>
>>>>>>>       >>>>>>> and be done with it until (say) the next release or
>>>>>>> next
>>>>>>>      released
>>>>>>>       >>>>>>> BSgenome.  I considered looping through the following
>>>>>>> BSgenomes
>>>>>>>       >>>>>>
>>>>>>>       >>>>>> myself...
>>>>>>>       >>>>>>>
>>>>>>>       >>>>>>> and if it isn't strongly opposed by (everyone) I may
>>>>>>> still
>>>>>>>      do exactly
>>>>>>>       >>>>>>> that.  Seems useful, no?
>>>>>>>       >>>>>>>
>>>>>>>       >>>>>>> e.g. for the following 42 builds,
>>>>>>>       >>>>>>>
>>>>>>>       >>>>>>> grep("(UCSC|NCBI)", unique(gsub(".masked", "",
>>>>>>>      available.genomes())),
>>>>>>>       >>>>>>> value=TRUE)
>>>>>>>       >>>>>>> [1] "BSgenome.Amellifera.UCSC.apiMel2"
>>>>>>>       >>>>>>
>>>>>>>       >>>>>> "BSgenome.Btaurus.UCSC.bosTau3"
>>>>>>>       >>>>>>>
>>>>>>>       >>>>>>>
>>>>>>>       >>>>>>> [3] "BSgenome.Btaurus.UCSC.bosTau4"
>>>>>>>       >>>>>>
>>>>>>>       >>>>>> "BSgenome.Btaurus.UCSC.bosTau6"
>>>>>>>       >>>>>>>
>>>>>>>       >>>>>>>
>>>>>>>       >>>>>>> [5] "BSgenome.Btaurus.UCSC.bosTau8"
>>>>>>>       >>>>>>> "BSgenome.Celegans.UCSC.ce10"
>>>>>>>       >>>>>>>
>>>>>>>       >>>>>>> [7] "BSgenome.Celegans.UCSC.ce2"
>>>>>>>        "BSgenome.Celegans.UCSC.ce6"
>>>>>>>       >>>>>>>
>>>>>>>       >>>>>>> [9] "BSgenome.Cfamiliaris.UCSC.canFam2"
>>>>>>>       >>>>>>> "BSgenome.Cfamiliaris.UCSC.canFam3"
>>>>>>>       >>>>>>> [11] "BSgenome.Dmelanogaster.UCSC.dm2"
>>>>>>>       >>>>>>> "BSgenome.Dmelanogaster.UCSC.dm3"
>>>>>>>       >>>>>>> [13] "BSgenome.Dmelanogaster.UCSC.dm6"
>>>>>>>       >>>>>>
>>>>>>>       >>>>>> "BSgenome.Drerio.UCSC.danRer5"
>>>>>>>       >>>>>>>
>>>>>>>       >>>>>>>
>>>>>>>       >>>>>>> [15] "BSgenome.Drerio.UCSC.danRer6"
>>>>>>>       >>>>>>
>>>>>>>       >>>>>> "BSgenome.Drerio.UCSC.danRer7"
>>>>>>>       >>>>>>>
>>>>>>>       >>>>>>>
>>>>>>>       >>>>>>> [17] "BSgenome.Ecoli.NCBI.20080805"
>>>>>>>       >>>>>>> "BSgenome.Gaculeatus.UCSC.gasAcu1"
>>>>>>>       >>>>>>> [19] "BSgenome.Ggallus.UCSC.galGal3"
>>>>>>>       >>>>>>
>>>>>>>       >>>>>> "BSgenome.Ggallus.UCSC.galGal4"
>>>>>>>       >>>>>>>
>>>>>>>       >>>>>>>
>>>>>>>       >>>>>>> [21] "BSgenome.Hsapiens.NCBI.GRCh38"
>>>>>>>       >>>>>>> "BSgenome.Hsapiens.UCSC.hg17"
>>>>>>>       >>>>>>>
>>>>>>>       >>>>>>> [23] "BSgenome.Hsapiens.UCSC.hg18"
>>>>>>>       >>>>>>> "BSgenome.Hsapiens.UCSC.hg19"
>>>>>>>       >>>>>>>
>>>>>>>       >>>>>>> [25] "BSgenome.Hsapiens.UCSC.hg38"
>>>>>>>       >>>>>>> "BSgenome.Mfascicularis.NCBI.5.0"
>>>>>>>       >>>>>>> [27] "BSgenome.Mfuro.UCSC.musFur1"
>>>>>>>       >>>>>>
>>>>>>>       >>>>>> "BSgenome.Mmulatta.UCSC.rheMac2"
>>>>>>>       >>>>>>>
>>>>>>>       >>>>>>>
>>>>>>>       >>>>>>> [29] "BSgenome.Mmulatta.UCSC.rheMac3"
>>>>>>>       >>>>>>
>>>>>>>       >>>>>> "BSgenome.Mmusculus.UCSC.mm10"
>>>>>>>       >>>>>>>
>>>>>>>       >>>>>>>
>>>>>>>       >>>>>>> [31] "BSgenome.Mmusculus.UCSC.mm8"
>>>>>>>       >>>>>>> "BSgenome.Mmusculus.UCSC.mm9"
>>>>>>>       >>>>>>>
>>>>>>>       >>>>>>> [33] "BSgenome.Ptroglodytes.UCSC.panTro2"
>>>>>>>       >>>>>>> "BSgenome.Ptroglodytes.UCSC.panTro3"
>>>>>>>       >>>>>>> [35] "BSgenome.Rnorvegicus.UCSC.rn4"
>>>>>>>       >>>>>>
>>>>>>>       >>>>>> "BSgenome.Rnorvegicus.UCSC.rn5"
>>>>>>>       >>>>>>>
>>>>>>>       >>>>>>>
>>>>>>>       >>>>>>> [37] "BSgenome.Rnorvegicus.UCSC.rn6"
>>>>>>>       >>>>>>> "BSgenome.Scerevisiae.UCSC.sacCer1"
>>>>>>>       >>>>>>> [39] "BSgenome.Scerevisiae.UCSC.sacCer2"
>>>>>>>       >>>>>>> "BSgenome.Scerevisiae.UCSC.sacCer3"
>>>>>>>       >>>>>>> [41] "BSgenome.Sscrofa.UCSC.susScr3"
>>>>>>>       >>>>>>
>>>>>>>       >>>>>> "BSgenome.Tguttata.UCSC.taeGut1"
>>>>>>>       >>>>>>>
>>>>>>>       >>>>>>>
>>>>>>>       >>>>>>>
>>>>>>>       >>>>>>>
>>>>>>>       >>>>>>>
>>>>>>>       >>>>>>> Am I insane for suggesting this?  It would make things
>>>>>>> a
>>>>>>> little
>>>>>>>       >>>>>>> easier
>>>>>>>       >>>>>>
>>>>>>>       >>>>>> for
>>>>>>>       >>>>>>>
>>>>>>>       >>>>>>> rtracklayer, most SummarizedExperiment and SE-derived
>>>>>>>      objects, blah,
>>>>>>>       >>>>>>
>>>>>>>       >>>>>> blah,
>>>>>>>       >>>>>>>
>>>>>>>       >>>>>>> blah...
>>>>>>>       >>>>>>>
>>>>>>>       >>>>>>>
>>>>>>>       >>>>>>> Best,
>>>>>>>       >>>>>>>
>>>>>>>       >>>>>>> --t
>>>>>>>       >>>>>>>
>>>>>>>       >>>>>>>
>>>>>>>       >>>>>>>
>>>>>>>       >>>>>>>
>>>>>>>       >>>>>>> Statistics is the grammar of science.
>>>>>>>       >>>>>>> Karl Pearson
>>>>>>>      <http://en.wikipedia.org/wiki/The_Grammar_of_Science>
>>>>>>>       >>>>>>
>>>>>>>       >>>>>>
>>>>>>>       >>>>>>         [[alternative HTML version deleted]]
>>>>>>>       >>>>>>
>>>>>>>       >>>>>> _______________________________________________
>>>>>>>       >>>>>> Bioc-devel at r-project.org
>>>>>>> <mailto:Bioc-devel at r-project.org>
>>>>>>>      mailing list
>>>>>>>       >>>>>> https://stat.ethz.ch/mailman/listinfo/bioc-devel
>>>>>>>       >>>
>>>>>>>       >>>
>>>>>>>       >>>     [[alternative HTML version deleted]]
>>>>>>>       >>>
>>>>>>>       >>> _______________________________________________
>>>>>>>       >>> Bioc-devel at r-project.org <mailto:Bioc-devel at r-project.org>
>>>>>>>      mailing list
>>>>>>>       >>> https://stat.ethz.ch/mailman/listinfo/bioc-devel
>>>>>>>       >>
>>>>>>>       >>
>>>>>>>       >> _______________________________________________
>>>>>>>       >> Bioc-devel at r-project.org <mailto:Bioc-devel at r-project.org>
>>>>>>>      mailing list
>>>>>>>       >> https://stat.ethz.ch/mailman/listinfo/bioc-devel
>>>>>>>       >>
>>>>>>>       >
>>>>>>>       > --
>>>>>>>       > Hervé Pagès
>>>>>>>       >
>>>>>>>       > Program in Computational Biology
>>>>>>>       > Division of Public Health Sciences
>>>>>>>       > Fred Hutchinson Cancer Research Center
>>>>>>>       > 1100 Fairview Ave. N, M1-B514
>>>>>>>       > P.O. Box 19024
>>>>>>>       > Seattle, WA 98109-1024
>>>>>>>       >
>>>>>>>       > E-mail: hpages at fredhutch.org <mailto:hpages at fredhutch.org>
>>>>>>>       > Phone: (206) 667-5791 <tel:%28206%29%20667-5791>
>>>>>>>       > Fax: (206) 667-1319 <tel:%28206%29%20667-1319>
>>>>>>>       >
>>>>>>>       >
>>>>>>>       > _______________________________________________
>>>>>>>       > Bioc-devel at r-project.org <mailto:Bioc-devel at r-project.org>
>>>>>>>      mailing list
>>>>>>>       > https://stat.ethz.ch/mailman/listinfo/bioc-devel
>>>>>>>
>>>>>>>
>>>>>>>
>>>>>> --
>>>>>> Hervé Pagès
>>>>>>
>>>>>> Program in Computational Biology
>>>>>> Division of Public Health Sciences
>>>>>> Fred Hutchinson Cancer Research Center
>>>>>> 1100 Fairview Ave. N, M1-B514
>>>>>> P.O. Box 19024
>>>>>> Seattle, WA 98109-1024
>>>>>>
>>>>>> E-mail: hpages at fredhutch.org
>>>>>> Phone:  (206) 667-5791
>>>>>> Fax:    (206) 667-1319
>>>>>>
>>>>>
>>>>> _______________________________________________
>>>>> Bioc-devel at r-project.org mailing list
>>>>> https://stat.ethz.ch/mailman/listinfo/bioc-devel
>>>>>
>>>>>
>>>>
>>>>
>>>> --
>>>> Gabriel Becker, Ph.D
>>>> Computational Biologist
>>>> Genentech Research
>>>>
>>>>          [[alternative HTML version deleted]]
>>>>
>>>> _______________________________________________
>>>> Bioc-devel at r-project.org mailing list
>>>> https://stat.ethz.ch/mailman/listinfo/bioc-devel
>>>>
>>>
>>>
>>>
>> _______________________________________________
>> Bioc-devel at r-project.org mailing list
>> https://stat.ethz.ch/mailman/listinfo/bioc-devel
>>
>>
> --
> Hervé Pagès
>
> Program in Computational Biology
> Division of Public Health Sciences
> Fred Hutchinson Cancer Research Center
> 1100 Fairview Ave. N, M1-B514
> P.O. Box 19024
> Seattle, WA 98109-1024
>
> E-mail: hpages at fredhutch.org
> Phone:  (206) 667-5791
> Fax:    (206) 667-1319
>
> _______________________________________________
> Bioc-devel at r-project.org mailing list
> https://stat.ethz.ch/mailman/listinfo/bioc-devel
>

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