[Bioc-devel] PhredQuality from Biostrings

[Christian Ruckert]

Am 10.06.2011 19:54, schrieb Martin Morgan:
> On 06/10/2011 08:01 AM, Christian Ruckert wrote:
>> Hi,
>>
>> I have written a function to read-in Roche SFF(Standard Flowgram Format)
>> files into R. Now I want to store the contents in standard Bioconductor
>> structures (e.q. sequences as DNAStringSet object). I have the quality
>> scores as a list of integer vectors. One list entry for each sequence.
>> The vector lengths correspond to the sequence lengths. The vectors
>> contain entries between 0 and 40 corresponding to the base quality at
>> this position.
>>
>
> Hi Christian
>
> Maybe along the lines of
>
> PhredQuality(sapply(qualitylist, function(x) rawToChar(as.raw(x + 33))))

This really speeds up things, thanks.

> or via ShortRead::readQual / readFastaQual (can use a character vector
> for the path; no need to create a RochePath). Probably you'll find
> ShortReadQ useful for coordinating the sequences and qualities
>
> Martin

I have successfully created a ShortReadQ object out of my sequences

 > srq
class: ShortReadQ
length: 95551 reads; width: 77..1201 cycles

Is it reasonable to use ShortReadQ for sequences from Roche with their 
differing lengths? It seems to work but the manual always states 
"uniform-length short reads".

Christian