[Bioc-devel] Dimension (rows & columns) in the writeCDF function seems to be swapped
Groot, Philip de
philip.degroot at wur.nl
Wed May 19 10:00:29 CEST 2010
I am contacting you because I am puzzled with an issue that is related to affxparser. I submitted this message to the bioc-devel list on april 29 (subject: "RE: [Bioc-devel] FW: problem in ReadAffy function (affy and affyio libraries)") but never received any reply. Interestingly, in the file writeCDF.R (affxparser source) the following comment is present:
# 2006-09-11 /HB
# o BUG FIX: nrows & ncols were swapped in the CDF header.
I succesfully created a "mogene11stv1cdf" library that can be used with the "affy" library. Benefit of this is that e.g. the affyPLM library can be used for creating informative plots. I applied rma utilizing affy and oligo and found exactly the same normalized intensities (see attached png-image).
So far so good, BUT... in order to get rma working for affy, I needed to switch the number or rows and columns when creating the CDF-file. And this puzzles me a lot! I do not understand where this originates from.
Let me explain. I use the oligo library and the "pd.mogene.1.1.st.v1" annotation file to create the CDF-file. I (together with Guido Hooiveld) adapted the original "PdInfo2Cdf.R" script for this purpose. Information on the original "PdInfo2Cdf.R" script is here: http://www.aroma-project.org/node/40. The adapted file is also in the attachment.
The CDF-file is created as follows:
PdInfo2Cdf("pd.mogene.1.1.st.v1", <An appropriate .CEL-file>);
make.cdf.package(file="pdmogene11stv1.cdf", packagename = "mogene11stv1cdf", author="Philip de Groot", maintainer="Philip de Groot <Philip.deGroot at wur.nl>", version="2.1.0", species="Mus_musculus")
Both CDF-files are available in a single zip-file via sendit:
Does somebody has an explanation for this? Is it a bug in writeCDF?
Dr. Philip de Groot Ph.D.
Wageningen University / TIFN
Nutrition, Metabolism & Genomics Group
Division of Human Nutrition
PO Box 8129, 6700 EV Wageningen
Visiting Address: Erfelijkheidsleer: De Valk, Building 304
Dreijenweg 2, 6703 HA Wageningen
E-mail: Philip.deGroot at wur.nl<mailto:Philip.deGroot at wur.nl>
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