## Abstract

Many variables of interest, such as experimental results, yields or
economic indicators, are naturally expressed as rates, proportions or
indices whose values are necessarily between zero and one or more
generally two fixed values known in advance. Beta regression allows us
to model these data with a great deal of flexibility, since the density
functions of the Beta laws can take on a wide variety of forms. However,
like all usual regression models, it cannot be applied directly when the
predictors present multicollinearity problems or worse when they are
more numerous than the observations. These situations are frequently
encountered in chemistry, medicine, economics or marketing. To
circumvent this difficulty, we formulate an extension of PLS regression
for Beta regression models. This, along with several tools such as
cross-validation and bootstrap techniques, is available for the
`R`

language in the `plsRbeta`

library.

### Keywords

Beta Regression, PLS Regression, PLS Beta Regression, Cross
validation, Bootstrap techniques, `R`

language

Classification/MSC: 62F40, 62J07, 62J12, 62P10, 62P20, 62P30

## Introduction

PLS regression, the result of the NIPALS algorithm initially
developed by Wold (Wold, 1966)^{1} and explained in detail by Tenenhaus
(Tenenhaus, 1998)^{2}, has already been successfully extended to
generalized linear models by Bastien *et al.* (Bastien, 2005)^{3} and to Cox
models by Bastien (Bastien 2008)^{4} and (Bastien 2015)^{5}.

We proposed an extension of PLS regression to Beta regression models
in Bertrand (2013)^{6}. Indeed, the practical interest of the Beta
distribution had been asserted several times, for example by Johnson
*et al.* (Johnson, 1995)^{7} :

“Beta distributions are very versatile and a variety of uncertanties can be usefully modelled by them. This flexibility encourages its empirical use in a wide range of applications.”

Several articles have focused on the study of Beta regression and its
properties. Let us mention in particular, the article by Ferrari and
Cribari-Neto (Ferrari, 2004)^{8} for an introduction to these models and
those by Kosmidis and Firth (Kosmidis, 2010)^{9}, Simas *et al.*
(Simas, 2010)^{10} and Grün *et al.* (Grün, 2012)^{11} for
extensions or improvements of the estimation techniques of these
models.

We will assume in the rest of the paper that the answer studied has values in the interval \([0;1]\). The model we propose can of course be used as soon as the answer \(Y\) has values in a bounded interval \([a;b]\), with \(a<b\) fixed and known, by studying \((Y-a)/(b-a)\) instead of \(Y\).

## PLS Beta Regression

### Beta Regression

When it is non-zero, the density function of the \(\textrm{Beta}(p,q)\) law is given by :
\[\begin{equation}
\pi(y;p;q)=\frac{\Gamma(p+q)}{\Gamma(p)\Gamma(q)}y^{p-1}(1-y)^{q-1},
\quad 0<y<1 \quad \textrm{(E.1)}
\end{equation}\] with \(p>0\), \(q>0\) and \(\Gamma(\cdot)\) the Euler gamma function.
If \(Y\) follows a \(\textrm{Beta}(p,q)\) distribution, its
expectation and variance are equal to : \[\begin{equation}
\mathbb{E}(Y)=\frac{p}{p+q} \quad \textrm{et} \quad
\mathrm{Var}(Y)=\frac{pq}{(p+q)^2(p+q+1)}\cdot \quad \textrm{(E.2)}
\end{equation}\] In order to be able to apply techniques similar
to those used for generalized linear models by McCullagh and Nelder,
Ferrari and Cribari-Neto (Ferrari, 2004)^{12} propose to rephrase
the Beta law as follows. By posing \(\mu=p/(p+q)\) and \(\phi=p+q\), i.e. \(p=\mu\phi\) and \(q=(1-\mu)\phi\), the Equation (\(\textrm{E.2}\)) becomes : \[
\mathbb{E}(Y)=\mu \quad \textrm{et} \quad
\mathrm{Var}(Y)=\frac{V(\mu)}{1+\phi}
\] where \(V(\mu)=\mu(1-\mu)\).
Thus \(\mu\) is the mean value of the
response and \(\phi\) can be
interpreted as a precision parameter since, for a fixed \(\mu\), the higher the value of \(\phi\), the smaller the variance of the
response. With these new parameters, the density function given in
Equation (\(\textrm{E.1}\)) is equal to
: \[\begin{equation}
\pi(y;\mu;\phi)=\frac{\Gamma(\phi)}{\Gamma(\mu\phi)\Gamma((1-\mu)\phi)}y^{\mu\phi-1}(1-y)^{(1-\mu)\phi-1},
\quad 0<y<1. \quad \textrm{(E.3)}
\end{equation}\]

It is not possible to apply directly the theory of the generalized
linear model, as introduced by the founding article of Nelder and
Wedderburn (Nelder, 1972)^{13} and then taken up again in the eponymous
book of McCullagh and Nelder (McCullagh, 1995)^{14}, because it is based
on the use of a family of laws put in natural exponential form (NEF).
However, as indicated by Morris (Morris, 1982)^{15}, the family of beta
laws, with the parameterization taken up by Ferrari and Cribari-Neto
(Ferrari, 2004)^{16} and introduced above, is certainly a
univariate exponential family but not a natural exponential family.
Indeed, the latter are characterized by their variance function. But the
variance function of this parameterization of the family of beta laws is
already that of the natural exponential family formed by the binomial
laws, hence the result. For the same reason, it is not possible to
characterize, within the exponential families, the family of Beta
distributions by means of their variance function.

Let \(Y_1\), \(\ldots\), \(Y_n\) be independent random variables distributed according to the density function given in Equation (\(\textrm{E.3}\)) of mean \(\mu_t\) and unknown precision \(\phi\).

We obtain the Beta regression model by assuming that the mean of \(Y_t\), \(1\leqslant t\leqslant n\), can be written : \[\begin{equation} g(\mu_t)=\sum_{i=1}^kx_{ti}\beta_i=\eta_k \end{equation}\] where \(\beta=(\beta_1,\ldots,\beta_k)'\) is a vector of unknown regression parameters (\(\beta \in \mathbb{R}^k\)), \('\) denoting the matrix transpose, and \(x_{t1,},\ldots,x_{tk}\) are the observations of the \(k\) predictors with \(k<n\) that are assumed to be known and fixed. Finally, \(g(\cdot)\) is a strictly monotone, twice derivable, surjective link function defined on the interval \(]0;1[\) and with values in \(\mathbb{R}\). The variance of \(Y_t\) is a function of \(\mu_t\) and thus depends on the value of the covariates. Therefore, the model automatically takes into account the possible lack of homoscedasticity.

There are several common choices for the link function \(g(\cdot)\). For example, the logit link
\(g(\mu)=\log{\mu/(1-\mu)}\), the
probit link \(g(\mu)=\Phi^{-1}(\mu)\),
with \(\Phi\) the distribution function
of the centered and reduced normal distribution, the complementary
log-log link \(g(\mu)=-\log(1-\mu))\),
the log-log link \(g(\mu)=-\log(-\log(\mu))\). A detailed
study of these links has been made by McCullagh and Nelder (McCullagh,
1995)^{17}
and Atkinson (Atkinson, 1985)^{18} has proposed others. As usual, the use of
the logit link makes it possible to interpret the exponential of the
coefficients of the covariates in terms of the odds ratio.

### PLS Regression

Consider the centered variables \(Y\), \(x_1\), , \(x_j\), , \(x_p\). Let \(X\) be the matrix of predictors \(x_1\), , \(x_j\), , \(x_p\). PLS regression is well known and
exhaustively described by Höskuldsson (Höskuldsson, 1988)^{19}, Wold *et
al.* (Wold, 2001)^{20} and Tenenhaus (Tenenhaus, 1998)^{21}. The
classical presentation of PLS regression is in algorithmic form. We will
only recall the elements that are useful for the following. PLS
regression is a non-linear model which allows to construct orthogonal
components \(t_h\) obtained by
maximizing the quantities \(cov(Y,t_h)\). Let \(T\) be the matrix formed by these
components, we have : \[\begin{eqnarray}
Y = Tc' + \epsilon,
\end{eqnarray}\] where \(\epsilon\) is the vector of residuals and
\(c'\) is the vector of component
coefficients. By positing \(T=XW^*\),
where \(W^*\) is the matrix of the
coefficients of the \(x_j\) variables
in each \(t_h\) component, we have the
direct expression for the response \(Y\) using the \(x_j\) predictors: \[\begin{eqnarray}
Y = X W^*c' + \epsilon.\quad (\textrm{E.4})
\end{eqnarray}\] Expanding the right-hand side of Equation~(\(\textrm{E.4}\)), we obtain for each \(Y_i\) component of \(Y\): \[\begin{eqnarray}
Y_i = \sum_{h=1}^H \left(c_hw_{1h}^*x_{i1} + c_hw_{ph}^*x_{ip} \right) +
\epsilon_i,
\end{eqnarray}\] \(H\) being the
number of components retained in the final model with \(H \leqslant \mathrm{rg}(X)\), \(H\) being in general much lower than the
rank of \(X\) and \(p\) being equal to the number of variables
contained in the matrix \(X\). The
coefficients \(c_hw^*_{jh}\), where
\(1 \leqslant j \leqslant p\),
following the notation with \(*\) of
Wold *et al.* (Wold, 2001)^{22}, translate the relation between the
vector \(Y\) and the variables \(x_j\) through the components \(t_h\).

### PLS Beta Regression

PLS Beta Regression of the response \(Y\) on the variables \(x_1\), , \(x_j\), , \(x_p\) with \(H\) components \(t_h=w^*_{1h}x_{i1}+\cdots+w^*_{ph}x_{ip}\) is written : \[\begin{eqnarray} g(\mu) = \sum_{h=1}^H \left(c_h \sum_{j=1}^p w_{jh}^*x_{ij}\right), \end{eqnarray}\] where \(mu\) is the expectation of \(Y\). The link \(g(\cdot)\) is to be chosen among the links logit, probit, complementary log-log, log-log, Cauchit and log according to the type of data and the quality of the model fit to the data.

The algorithm for determining the PLS \(t_h\) components of a Beta PLS regression model is as follows:

- Compute the first PLS component \(t_1\) :

- Compute the coefficient \(a_{1j}\) of \(x_j\) in the Beta regression of \(Y\) on \(x_j\) for each predictor \(x_j\), \(1 \leqslant j \leqslant p\).
- Norm the column vector \(a_1\) : \(w_1=a_1/\| a_1\|\).
- Compute the component \(t_1=1/(w_1'w_1)Xw_1\).

- Compute the second PLS component \(t_2\) :

- Compute the coefficient \(a_{2j}\) of \(x_j\) in the Beta regression of \(Y\) on \(t_1\) and \(x_j\) for each predictor \(x_j\), \(1 \leqslant j \leqslant p\).
- Norm the column vector \(a_2\) : \(w_2=a_2/\| a_2\|\).
- Compute the residual matrix \(X_1\) of the linear regression of \(X\) on \(t_1\).
- Compute the component \(t_2=1/(w_2'w_2)X_1w_2\).
- Express the component \(t_2\) in terms of predictors \(X\): \(t_2=Xw^*_2\).

- We assume having constructed \(h-1\) components \(t_1\), \(t_{h-1}\). We then compute the \(h-\)th PLS component \(t_h\):

- Compute the coefficient \(a_{hj}\) of \(x_j\) in the Beta regression model of \(Y\) on \(t_1\), \(t_2\), $t_ldots, \(t_{h-1}\), and \(x_j\) for each predictor \(x_j\), \(1 \leqslant j \leqslant p\).
- Normalize the column vector \(a_h\) : \(w_h=a_h/\| a_h\|\).
- Compute the residual matrix \(X_{h-1}\) of the linear regression of \(X\) on \(t_1\), \(t_2\), \(t_{h-1}\).
- Compute the component \(t_h=1/(w_h'w_h)X_{h-1}w_h\).
- Express the component \(t_h\) in terms of predictors \(X\): \(t_h=Xw^*_h\).

##### Proposition

The PLS components \((t_h)_{1 \leqslant h \leqslant H}\) are orthogonal.

It is easy to modify the previous algorithm to be able to handle
incomplete datasets (Tenenhaus, 1998)^{23}.

## Bootstrap techniques, cross-validation and software implementation

### Bootstrap techniques

We assume that we have retained the appropriate number \(m\) of components of a \(Y\) Beta PLS regression model on \(x_1\), , \(x_j\), , \(x_p\). We propose the following algorithm to construct confidence intervals and significance tests for the predictors \(x_j\), \(1 \leqslant j \leqslant p\), using bootstrap techniques. Let \(\widehat{F}_{(T|Y)}\) be the empirical distribution function given the matrix \(T\) formed by the \(m\) PLS components and the response \(Y\).

*Step 1.*Draw \(B\) samples from \(\widehat{F}_{(T|Y)}\).*Step 2.*For all \(b=1,\ldots,B\), calculate : \[ c^{(b)}=(T^{(b)}{}'T^{(b)})^{-1}T^{(b)}{}'Y^{(b)} \quad \mbox{and} \quad b^{(b)}=W^*{c'}^{(b)}, \] where \([T^{(b)},Y^{(b)}]\) is the \(b-\)th bootstrap sample, \({c'}^{(b)}\) is the vector of component coefficients and \(b^{(b)}\) is the vector of coefficients of the original \(p\) predictors for this sample and finally \(W^*\) is the fixed matrix of the weights of the predictors in the original model with \(m\) components.*Step 3.*For each \(j\), let \(\phi_{b_j}\) be the Monte Carlo approximation of the distribution function of the bootstrap statistic of \(b_j\). For each \(b_j\), whisker boxes and confidence intervals can be constructed using the percentiles of \(\Phi_{b_j}\). A confidence interval can be defined as \(I_j(\alpha)=]\Phi^{-1}_{b_j}(\alpha), \Phi^{-1}_{b_j}(1-\alpha)[\) where \(\Phi^{-1}_{b_j}(\alpha)\) and \(\Phi^{-1}_{b_j}(1-\alpha)\) are the values obtained from the distribution function of the bootstrap statistic such that a nominal level of confidence of level \(100(1-2\alpha)\%\) is reached. In order to improve the quality of the confidence interval in terms of coverage rates, i.e., the ability of \(I_j(\alpha)\) to provide the expected coverage rates, it is possible to use several construction techniques: normal, percentile, or \(BC_a\) (Efron 1993)^{24}or (Davison 1997)^{25}. The resulting intervals are not intended to be used for multiple or pairwise comparisons and should be interpreted separately.

### Strengths of the software implementation

The `plsRbeta`

function library for the `R`

language implements PLS Beta regression models. It uses the Beta
regression implemented in the `betareg`

(Cribari-Neto,
2010)^{26}
function library for the `R`

language to perform the
step.

- Beta PLS regression models with complete or incomplete data.
- Choice of the number of components thanks to different criteria AIC, BIC, modified \(R^2\), stop of significance of the \(t_{m+1}\) component when none of the \(a_{m+1}\) coefficients is significant in the model at a given \(alpha'\) level or by using a \(Q^2\) criterion estimated by cross validation.
- Repeated \(k\)-fold cross-validation with complete or incomplete data. Tables 3 and 5 are examples of using cross-validation to determine the appropriate number of components.
- Bootstrapping predictor coefficients for Beta PLS regression models
with complete or incomplete data. Various interval constructions,
detailed in Efron and Tibshirani (Efron, 1993)
^{27}or Davison and Hinkley (Davison, 1997)^{28}, are available and are based on the`boot`

(Canty, 2021)^{29}function library for the`R`

language. Figure 5 is an example of whisker boxes constructed from the bootstrap distribution of the coefficients of the predictors of a PLS Beta regression model. Figures 2, 3, and 6 are examples of using bootstrap techniques to establish the significance of the predictors of a PLS Beta regression model.

### Selecting the number of components

A crucial problem for the correct use of PLS regression is the
determination of the number of components. If, in the case of the
original PLS regression, the \(Q^2\)
criterion is extremely efficient, its good properties unfortunately
disappear for the PLS generalized linear regression models. A simulation
study is therefore necessary to determine a functional criterion to
choose the number of components. We propose to compare the following
criteria \(AIC\) and \(BIC\) (Cribari-Neto, 2010)^{30}, Pearson’s \(\chi^2\), Pearson’s \(R^2\) and pseudo-\(R^2\) (Ferrari, 2004)^{31} or cumulative \(Q^2\chi^2\) and \(Q^2\chi^2\) criteria estimated by 5-group
cross-validation (5-CV) or 10-group cross-validation (10-CV) (Bastien,
2005)^{32}.

The following parameters were used for the simulation design.

- Number of individuals: 25, 50 and 100.
- Number of variables: 10, 25, 50 and 100.
- Number of components: 2, 4 and 6.
- \(\phi\) dispersion : 2,5, 5, 10 and 15.

The algorithm used to create the simulated data is a direct
adaptation of the algorithm of Li *et al.* (Li, 2002)^{33} which
is itself a multivariate generalization of that of Naes and Martens
(Naes, 1985)^{34}. This type of generalization has already
been used successfully in the case of PLS logistic regression models
(Meyer, 2010)^{35}.

Table 1 is an example of a result for 25 individuals, 10 variables, 2
components and a dispersion parameter \(\phi\) equal to 2.5. For 100 simulated data
sets, a maximum number of 6 components had to be calculated. The average
number of components that could actually be computed, as well as the
number of complete failures of the beta regression model fit are shown
in Table 2. The abbreviations \(ML\),
\(BR\), and \(BC\) indicate that the Beta regressions
used to fit the Beta PLS regression are based on maximum likelihood,
bias reduction, or bias correction (Kosmidis, 2010)^{36}. The prefixes \(K5\) and \(K10\) mean that the value was obtained
after cross-validation in \(K=5\) or
\(K=10\) groups.

Criterion | Mean | SD | Median | MAD | Criterion | Mean | SD | Median | MAD |
---|---|---|---|---|---|---|---|---|---|

\(ML.AIC\) | 3.53 | 0.94 | 3.5 | 0.74 | \(K5.ML.\chi^2\) | 2.86 | 2.05 | 2 | 1.48 |

\(ML.BIC\) | 3.1 | 0.8 | 3 | 0 | \(K10.ML.Q^2\chi^2_{\textrm{cum}}\) | 4.71 | 1.97 | 6 | 0 |

\(ML.\chi^2\) | 2.84 | 2.05 | 2 | 1.48 | \(K10.ML.Q^2\chi^2\) | 0 | 0 | 0 | 0 |

\(ML.RSS\) | 5.15 | 1.31 | 6 | 0 | \(K10.ML.\textrm{pre}\chi^2\) | 1.37 | 0.49 | 1 | 0 |

\(ML.\textrm{pseudo-}R^2\) | 3.96 | 1.59 | 4 | 2.97 | \(K10.ML.\chi^2\) | 2.84 | 2.05 | 2 | 1.48 |

\(ML.R^2\) | 5.15 | 1.31 | 6 | 0 | \(K5.BC.Q^2\chi^2_{\textrm{cum}}\) | 4.64 | 1.98 | 6 | 0 |

\(BC.AIC\) | 3.42 | 0.87 | 3 | 1.48 | \(K5.BC.Q^2\chi^2\) | 0.06 | 0.24 | 0 | 0 |

\(BC.BIC\) | 3.02 | 0.79 | 3 | 0 | \(K5.BC.\textrm{pre}\chi^2\) | 1.48 | 0.5 | 1 | 0 |

\(BC.\chi^2\) | 5.3 | 1.57 | 6 | 0 | \(K5.BC.\chi^2\) | 2.89 | 2.04 | 2 | 1.48 |

\(BC.RSS\) | 5.14 | 1.33 | 6 | 0 | \(K10.BC.Q^2\chi^2_{\textrm{cum}}\) | 4.84 | 1.87 | 6 | 0 |

\(BC.\textrm{pseudo-}R^2\) | 3.98 | 1.58 | 4 | 2.97 | \(K10.BC.Q^2\chi^2\) | 0.07 | 0.26 | 0 | 0 |

\(BC.R^2\) | 5.14 | 1.33 | 6 | 0 | \(K10.BC.\textrm{pre}\chi^2\) | 1.54 | 0.5 | 2 | 0 |

\(BR.AIC\) | 3.43 | 0.81 | 3 | 1.48 | \(K10.BC.\chi^2\) | 2.87 | 2.04 | 2 | 1.48 |

\(BR.BIC\) | 3.04 | 0.74 | 3 | 0 | \(K5.BR.Q^2\chi^2_{\textrm{cum}}\) | 4.42 | 2.02 | 6 | 0 |

\(BR.\chi^2\) | 5.16 | 1.64 | 6 | 0 | \(K5.BR.Q^2\chi^2\) | 0.07 | 0.26 | 0 | 0 |

\(BR.RSS\) | 5.03 | 1.28 | 6 | 0 | \(K5.BR.\textrm{pre}\chi^2\) | 1.47 | 0.5 | 1 | 0 |

\(BR.\textrm{pseudo-}R^2\) | 3.95 | 1.53 | 4 | 1.48 | \(K5.BR.\chi^2\) | 2.89 | 2.06 | 2 | 1.48 |

\(BR.R^2\) | 5.03 | 1.28 | 6 | 0 | \(K10.BR.Q^2\chi^2_{\textrm{cum}}\) | 4.53 | 1.94 | 6 | 0 |

\(K5.ML.Q^2\chi^2_{\textrm{cum}}\) | 4.69 | 1.89 | 6 | 0 | \(K10.BR.Q^2\chi^2\) | 0.08 | 0.27 | 0 | 0 |

\(K5.ML.Q^2\chi^2\) | 0 | 0 | 0 | 0 | \(K10.BR.\textrm{pre}\chi^2\) | 1.52 | 0.5 | 2 | 0 |

\(K5.ML.\textrm{pre}\chi^2\) | 1.43 | 0.5 | 1 | 0 | \(K10.BR.\chi^2\) | 2.87 | 2.04 | 2 | 1.48 |

Note that the MAD is the median of the absolute deviations from the median. It is a robust indicator of dispersion naturally associated with the median. A value of 0 for the MAD means that in more than half of the simulations, the value chosen for the number of components is equal to the median value of all simulations.

In general, the results of the simulation study show that the \(Q^2\chi^2\) (5-CV and 10-CV), already known
for its surprising behavior in PLS logistic regression (Bastien, 2005)^{37},
(Meyer, 2010)^{38}, does not behave much better for the Beta
PLS regression models. Maximizing the \(R^2\) or pseudo-\(R^2\) criteria, also proves inefficient.
The \(AIC\) and \(BIC\) criteria systematically retain a few
too many components. This tendency is also known in the case of the
traditional PLS Regression (Kramer, 2011)^{39} as well as in the
case of the Logistic PLS Regression (Meyer, 2010)^{40}.

Mean | SD | Median | MAD | Failures | |
---|---|---|---|---|---|

ML | 5.55 | 1.22 | 6 | 0 | 0 |

BC | 5.52 | 1.24 | 6 | 0 | 0 |

BR | 5.43 | 1.22 | 6 | 0 | 1 |

ML (5-CV) | 5.59 | 1.17 | 6 | 0 | 1 |

ML (10-CV) | 5.55 | 1.22 | 6 | 0 | 0 |

BC (5-CV) | 5.63 | 1.08 | 6 | 0 | 2 |

BC (10-CV) | 5.6 | 1.13 | 6 | 0 | 1 |

BR (5-CV) | 5.62 | 1.09 | 6 | 0 | 4 |

BR (10-CV) | 5.6 | 1.13 | 6 | 0 | 1 |

We find that the proposed PLS Beta Regression algorithm succeeds in extracting the maximum number of components requested very consistently and that the fit is impossible in only one of the 100 simulations for the BR technique. The cross-validation, on the other hand, is completed in 98.5 % of the simulations.

## Example of application in medicine

Cancerous tumors represent one of the three main causes of death in the Western world. The understanding of the mechanisms of cancer pathologies is currently based on the study of the interrelationships of acquired genetic abnormalities, which appear in tissues during the process of cancerization. These abnormalities are frequently analyzed by allelotyping, allowing to determine for a more or less important number of gene sites, the presence or not of a modification of the number of copies of each gene. The multivariate description of these anomalies is informative about the carcinogenesis process. Furthermore, the set of these gene sites with or without abnormalities can be used to try to predict certain clinical or biological characteristics of the tumor such as the tumor cell count on biopsy of a lesion. Modeling in a statistical model of rate, variable whose space of variation is contained in the closed interval \([0;1]\) as predicted variable suggests the use of Beta regression. Furthermore, allelotyping data are characterized by frequent collinearity and a large proportion of missing data. Moreover, the data matrix often has dimensions \((i;j)\) such that \(j>i\), which makes the matrix non-invertible, posing difficulties in fitting a regression model. The PLS Beta regression that we have developed is therefore particularly well suited to deal with allelotyping data in the particular context of predicting a rate variable.

The example is that of allelotyping data obtained on a series of 93 patients with different types of lung cancer and with \(23.2 \%\) missing values. The predicted variable is the tumor cellularity rate of the intraoperative tumor specimen. The explanatory variables are composed of 56 binary variables indicating the presence of an abnormality on each of the 56 microsatellites and three clinical variables.

The selection of variables is very important in this example because it allows to define a subset of predictors, i.e. gene sites, able to predict the tumor cell rate. Indeed, cancer pathologies are acquired genetic pathologies and some of these anomalies are the cause and others the consequence of the tumor pathology. Moreover, the information contained in the different microsatellite gene sites is potentially redundant. Variable selection, separating variables that probably play a driving role in tumor development from variables that merely reflect random background noise induced by abnormalities caused by tumor development, is therefore an indispensable aid to understanding the underlying mechanisms of tumorigenesis.

Table 3 summarizes the values of different criteria used to determine the number of components to be used to properly model the allelotype data with BR and a logit link.

Nb of Comp. | 0 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 |
---|---|---|---|---|---|---|---|---|---|---|

AIC | \(-23.9\) | \(-48.2\) | \(-63.4\) | \(-76.2\) | \(-90.2\) | \(-101.5\) | \(-114.2\) | \(-116.9\) | \(-120.5\) | \(\mathbf{-121.6}\) |

BIC | \(-18.5\) | \(-40.2\) | \(-52.7\) | \(-62.9\) | \(-74.2\) | \(-82.8\) | \(-92.9\) | \(-92.9\) | \(\mathbf{-93.8}\) | \(-92.3\) |

Pred Sig | \(15\) | \(7\) | \(1\) | \(2\) | \(0\) | \(\mathbf{2}\) | \(0\) | \(0\) | \(0\) | |

\(Q^2\chi^2\) (5-CV) | \(\mathbf{-0.3}\) | \(-1.0\) | \(-1.8\) | \(-2.8\) | \(-3.8\) | \(-5.9\) | \(-8.9\) | \(-10.4\) | \(-8.0\) | |

\(\chi^2\) Pearson | \(98.3\) | \(97.2\) | \(96.0\) | \(\mathbf{100.0}\) | \(96.8\) | \(91.5\) | \(89.1\) | \(88.6\) | \(87.4\) | \(86.4\) |

pseudo-\(R^2\) | \(0.21\) | \(0.31\) | \(0.40\) | \(0.47\) | \(0.53\) | \(0.58\) | \(0.59\) | \(0.61\) | \(\mathbf{0.62}\) | |

\(R^2\) Pearson | \(0.22\) | \(0.35\) | \(0.41\) | \(0.50\) | \(0.57\) | \(0.64\) | \(0.65\) | \(0.67\) | \(\mathbf{0.68}\) |

The stopping criterion as soon as there is no significant predictor would lead us to choose 6 components. This choice is confirmed by the pseudo-\(R^2\) or \(R^2\) criteria for which a kink appears from 6 components.

The BIC criterion invites us to choose 8 components and the AIC criterion an even higher number. During the simulation study, we noticed that these criteria are liberal and generally retain a few too many components.

Depending on the criteria, 6 or 8 components should be retained for a logit link. Taking into account the previous simulation study, we decide to retain 6 components. The same study was performed with the BC method or a log-log link and leads to the same conclusions.

Confidence intervals are then obtained for each of the predictors using bootstrap samples of size 1000 and the normal, basic, percentile or \(BC_a\) techniques.

The use of bootstrap techniques to establish the significance of the predictors of a PLS Beta regression model is illustrated in Figure 2 for the case of the 6-component model with the BR bias reduction technique.

In the end, we use the \(BC_a\) technique, known for its good properties, to select the variables that are significant at the 5 % threshold by retaining those for which the \(BC_a\) confidence interval does not contain 0, as shown in Figure 3 for the case of the 6-component model with the BR bias reduction technique.

Thus, we retain the variables in Table 4 as having a significant effect on the tumor cellularity rate of the intraoperative tumor specimen. We find that the two techniques for dealing with the Beta regression bias have results that differ only in the single variable EGF3, significant at the \(5 \%\) threshold for the BC technique and not for the BR technique.

Nb Comp | Significant Variables | |||||
---|---|---|---|---|---|---|

6 components | P4 | C3M | RB | FL7A | W2 | W4 |

MT4 | HLA | HLD | HLB | EA3 | EA2 | |

Bias Correction | EGF2 | EGF3 | FL7B | VSFGFR3 | VSTOP1 | VSTOP2A |

VSEGFR | AFRAEGFR | SRXRA | SMT | SHL | SEB | |

6 components | P4 | C3M | RB | FL7A | W2 | W4 |

MT4 | HLA | HLD | HLB | EA3 | EA2 | |

Bias Reduction | EGF2 | FL7B | VSFGFR3 | VSTOP1 | VSTOP2A | VSEGFR |

AFRAEGFR | SRXRA | SMT | SHL | SEB |

Figure 4 allows us to evaluate the stability of the significant variables selected by the bootstrap technique \(BC_a\) for a number of components varying from 1 to 6 and the two techniques BC and BR for fitting the Beta regression model.

```
library(plsRglm)
library(plsRbeta)
```

```
data(TxTum)
<- bootplsbeta(plsRbeta(formula=I(CELTUMCO/100)~.,data=TxTum,nt=6,modele="pls-beta",type="BR"), sim="ordinary", stype="i", R=1000)
TxTum.mod.bootBR6 #save(TxTum.mod.bootBR6,file="TxTum.mod.bootBR6.Rdata")
```

```
data("TxTum.mod.bootBR6")
<- suppressWarnings(plsRglm::confints.bootpls(TxTum.mod.bootBR6)) temp.ci
```

`boxplots.bootpls(TxTum.mod.bootBR6,indices = 2:nrow(temp.ci))`

`::plots.confints.bootpls(temp.ci,prednames=TRUE,indices = 2:nrow(temp.ci)) plsRglm`

```
<- (temp.ci[,7]<0&temp.ci[,8]<0)|(temp.ci[,7]>0&temp.ci[,8]>0)
ind_BCa_nt6BR rownames(temp.ci)[ind_BCa_nt6BR]
#> [1] "P4" "C3M" "RB" "FL7A" "W2" "W4"
#> [7] "MT4" "HLA" "HLD" "HLB" "EA3" "EA2"
#> [13] "EGF2" "FL7B" "VSFGFR3" "VSTOP1" "VSTOP2A" "VSEGFR"
#> [19] "AFRAEGFR" "SRXRA" "SMT" "SHL" "SEB"
```

`::plots.confints.bootpls(temp.ci,prednames=TRUE,indices = (2:nrow(temp.ci))[ind_BCa_nt6BR[-1]],articlestyle=FALSE,legendpos="topright") plsRglm`

```
#TxTum <- read.table("MET_rev_edt.txt",sep="\t",header=TRUE)
data("TxTum.mod.bootBC1")
<- plsRglm::confints.bootpls(TxTum.mod.bootBC1)
temp.ci
data("ind_BCa_nt1BC")
data("ind_BCa_nt2BC")
data("ind_BCa_nt3BC")
data("ind_BCa_nt4BC")
data("ind_BCa_nt5BC")
data("ind_BCa_nt6BC")
data("ind_BCa_nt1BR")
data("ind_BCa_nt2BR")
data("ind_BCa_nt3BR")
data("ind_BCa_nt4BR")
data("ind_BCa_nt5BR")
data("ind_BCa_nt6BR")
rownames(temp.ci)[ind_BCa_nt1BC]
#> [1] "P4" "E1" "RB" "P53" "HLA" "EA2" "F3B" "VSEGFR"
#> [9] "SHL" "SEB"
colnames(TxTum.mod.bootBC1$data)[-1])[]
(#> [1] "age" "sexe" "HISTOADK" "H2" "P3" "P4"
#> [7] "E1" "P5" "R10" "C3M" "P6" "RB"
#> [13] "FL7A" "P53" "W2" "P2" "P1" "W4"
#> [19] "MT1" "MT2" "MT4" "MT3" "HLA" "HLD"
#> [25] "HLC" "HLB" "EA1" "EA3" "EA2" "EA4"
#> [31] "EB1" "EB2" "EB3" "EB4" "EGF1" "EGF2"
#> [37] "EGF3" "EGF4" "EGF5" "EGF6" "FL7B" "VSFGF7"
#> [43] "F3A" "F3B" "VSFGFR3" "F4" "Q5" "VSTOP1"
#> [49] "VSTOP2A" "VSEGFR" "AFRAEGFR" "SRXRA" "SMT" "QMTAMPN"
#> [55] "QMTDELN" "SHL" "SEA" "SEB" "QPCRFGF7"
<- cbind(ind_BCa_nt1BC,
indics
ind_BCa_nt2BC,
ind_BCa_nt3BC,
ind_BCa_nt4BC,
ind_BCa_nt5BC,
ind_BCa_nt6BC,
ind_BCa_nt1BR,
ind_BCa_nt2BR,
ind_BCa_nt3BR,
ind_BCa_nt4BR,
ind_BCa_nt5BR,
ind_BCa_nt6BR)
<- rbind(colSums(indics[,1:6]),colSums(indics[,7:12]))
nbpreds colnames(nbpreds) <- c("1","2","3","4","5","6")
rownames(nbpreds) <- c("BC","BR")
```

```
nbpreds#> 1 2 3 4 5 6
#> BC 10 7 11 15 23 24
#> BR 9 7 9 15 21 23
```

```
<- indics[rowSums(indics)>0,c(1,7,2,8,3,9,4,10,5,11,6,12)]
inone <- t(apply(inone,1,as.numeric))
inone rownames(inone)
#> [1] "age" "P4" "E1" "C3M" "RB" "FL7A"
#> [7] "P53" "W2" "P2" "W4" "MT4" "HLA"
#> [13] "HLD" "HLB" "EA3" "EA2" "EB4" "EGF2"
#> [19] "EGF3" "EGF4" "FL7B" "F3B" "VSFGFR3" "VSTOP1"
#> [25] "VSTOP2A" "VSEGFR" "AFRAEGFR" "SRXRA" "SMT" "SHL"
#> [31] "SEA" "SEB"
colnames(inone) <- c("BC1","BR1","BC2","BR2","BC3","BR3","BC4","BR4","BC5","BR5","BC6","BR6")
colnames(inone) <- c("1 BC"," BR","2 BC"," BR","3 BC"," BR","4 BC"," BR","5 BC"," BR","6 BC"," BR")
```

```
inone#> 1 BC BR 2 BC BR 3 BC BR 4 BC BR 5 BC BR 6 BC BR
#> age 0 0 0 0 0 0 1 1 0 0 0 0
#> P4 1 1 1 1 1 1 1 1 1 1 1 1
#> E1 1 1 1 1 1 1 1 1 0 0 0 0
#> C3M 0 0 0 0 0 0 1 1 1 1 1 1
#> RB 1 1 1 1 1 1 1 1 1 1 1 1
#> FL7A 0 0 1 1 1 0 1 1 1 1 1 1
#> P53 1 1 1 1 1 1 1 1 1 1 0 0
#> W2 0 0 0 0 0 0 0 0 1 1 1 1
#> P2 0 0 0 0 1 1 0 0 0 0 0 0
#> W4 0 0 0 0 0 0 1 1 1 1 1 1
#> MT4 0 0 0 0 0 0 0 0 1 0 1 1
#> HLA 1 0 0 0 1 1 1 1 1 1 1 1
#> HLD 0 0 0 0 1 1 1 1 1 1 1 1
#> HLB 0 0 0 0 0 0 0 0 1 1 1 1
#> EA3 0 0 0 0 0 0 0 0 1 1 1 1
#> EA2 1 1 1 1 1 1 1 1 1 1 1 1
#> EB4 0 1 0 0 0 0 0 0 0 0 0 0
#> EGF2 0 0 0 0 0 0 0 0 1 0 1 1
#> EGF3 0 0 0 0 0 0 0 0 0 0 1 0
#> EGF4 0 0 0 0 0 0 1 1 0 0 0 0
#> FL7B 0 0 0 0 0 0 0 0 1 1 1 1
#> F3B 1 1 0 0 0 0 0 0 0 0 0 0
#> VSFGFR3 0 0 0 0 0 0 0 0 1 1 1 1
#> VSTOP1 0 0 0 0 0 0 0 0 1 1 1 1
#> VSTOP2A 0 0 0 0 1 1 1 1 1 1 1 1
#> VSEGFR 1 1 0 0 0 0 1 1 1 1 1 1
#> AFRAEGFR 0 0 0 0 0 0 1 1 1 1 1 1
#> SRXRA 0 0 0 0 0 0 0 0 1 1 1 1
#> SMT 0 0 0 0 0 0 0 0 1 1 1 1
#> SHL 1 0 0 0 1 0 0 0 0 0 1 1
#> SEA 0 0 1 1 0 0 0 0 0 0 0 0
#> SEB 1 1 0 0 0 0 0 0 1 1 1 1
```

```
library(bipartite)
#> Le chargement a nécessité le package : vegan
#> Le chargement a nécessité le package : permute
#> Le chargement a nécessité le package : lattice
#> This is vegan 2.5-7
#> Le chargement a nécessité le package : sna
#> Le chargement a nécessité le package : statnet.common
#>
#> Attachement du package : 'statnet.common'
#> Les objets suivants sont masqués depuis 'package:base':
#>
#> attr, order
#> Le chargement a nécessité le package : network
#>
#> 'network' 1.17.1 (2021-06-12), part of the Statnet Project
#> * 'news(package="network")' for changes since last version
#> * 'citation("network")' for citation information
#> * 'https://statnet.org' for help, support, and other information
#> sna: Tools for Social Network Analysis
#> Version 2.6 created on 2020-10-5.
#> copyright (c) 2005, Carter T. Butts, University of California-Irvine
#> For citation information, type citation("sna").
#> Type help(package="sna") to get started.
#> This is bipartite 2.16.
#> For latest changes see versionlog in ?"bipartite-package". For citation see: citation("bipartite").
#> Have a nice time plotting and analysing two-mode networks.
#>
#> Attachement du package : 'bipartite'
#> L'objet suivant est masqué depuis 'package:vegan':
#>
#> nullmodel
::visweb(t(inone),type="None",labsize=2,square="b",box.col="grey25",pred.lablength=7) bipartite
```

## Example of application in chemometrics

The goal is to find compounds that can predict the rate of cancer
cell infiltration in patients from spectrometry data. A healthy patient
has 0 % of cancer cells in a biopsy while a sick patient will have in a
biopsy a % higher than the sample contains cancer cells. The interest of
this experiment is to try to reduce considerably the time of analysis of
the biopsies by avoiding the need for a counting by a doctor specialized
in anatomy and pathological cytology. An additional statistical
difficulty appears here: there are more variables (180) than individuals
(80). More details on the experimental protocol followed are available
in the article in which this dataset has already been published (Piotto,
2012)^{41}.

We begin by determining the number of components to use to fit a model with all areas of the spectrum as explanatory variables. Table 5 contains the components for selecting the number of components. We again see the tendency of the AIC and BIC criteria to retain a very high number of components, namely more than 10. On the other hand, in this example the \(Q^2\chi^2\) criterion, estimated using a 10-group cross-validation (10-CV), seems relevant and invites us to keep 3 components. The same is true for the Pearson \(chi^2\). The pseudo-\(R^2\) is also in line with this since we observe a rapid decrease in the explanatory contribution following the addition of additional components beyond the third one.

Nb of Comp. | 0 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
---|---|---|---|---|---|---|---|---|---|---|---|

AIC | \(-32.35\) | \(-110.26\) | \(-128.86\) | \(-141.93\) | \(-152.50\) | \(-165.93\) | \(-183.71\) | \(-193.49\) | \(-206.75\) | \(-217.21\) | \(\mathbf{-231.30}\) |

BIC | \(-27.58\) | \(-103.11\) | \(-119.33\) | \(-130.02\) | \(-138.21\) | \(-149.25\) | \(-164.66\) | \(-172.05\) | \(-182.93\) | \(-191.01\) | \(\mathbf{-202.71}\) |

\(Q^2\chi^2\) (10-CV) | \(-1.05\) | \(-2.68\) | \(\mathbf{-1.00}\) | \(-1.51\) | \(-2.34\) | \(-3.71\) | \(-8.04\) | \(-211.19\) | \(-5.16E3\) | \(-4.16E4\) | |

\(\chi^2\) Pearson | \(76.78\) | \(76.44\) | \(\mathbf{80.58}\) | \(76.20\) | \(81.95\) | \(81.08\) | \(74.06\) | \(76.39\) | \(72.62\) | \(73.21\) | \(71.82\) |

pseudo-\(R^2\) | \(0.70\) | \(0.78\) | \(0.82\) | \(0.85\) | \(0.87\) | \(0.89\) | \(0.90\) | \(0.92\) | \(0.93\) | \(\mathbf{0.94}\) | |

\(R^2\) Pearson | \(0.57\) | \(0.64\) | \(0.73\) | \(0.75\) | \(0.81\) | \(0.86\) | \(0.88\) | \(0.90\) | \(0.92\) | \(\mathbf{0.94}\) |

We then construct bootstrap samples of size 250 of the predictor
coefficients for a 3-component model with the bias reduction (BR)
method. Figure 5 gives the whisker boxes of these distributions.
Confidence intervals are then obtained for each of the predictors with
the normal, basic, percentile or \(BC_a\) techniques. In the end, we use the
\(BC_a\) technique, known for its good
properties (Diciccio, 1996)^{42}, to select the significant variables at
the 5 % threshold by retaining those for which the \(BC_a\) confidence interval does not contain
0 as illustrated by Figure 6 for the case of the 3-component model with
the BR bias reduction technique.

```
data(colon)
<- colon orig
```

```
<- bootplsbeta(plsRbeta(X..Cellules.tumorales~.,data=colon,nt=3,modele="pls-beta"), sim="ordinary", stype="i", R=250)
modpls.boot3 #save(modpls.boot3,file="modpls.boot_nt3.Rdata")
```

```
data("modpls.boot_nt3")
<- suppressWarnings(plsRglm::confints.bootpls(modpls.boot3))
temp.ci <- (temp.ci[,7]<0&temp.ci[,8]<0)|(temp.ci[,7]>0&temp.ci[,8]>0)
ind_BCa_nt3 #save(ind_BCa_nt3,file="ind_BCa_nt3.Rdata")
```

`boxplots.bootpls(modpls.boot3,indices = 2:nrow(temp.ci))`

`::plots.confints.bootpls(temp.ci,prednames=TRUE,indices = 2:nrow(temp.ci)) plsRglm`

```
data(file="ind_BCa_nt3")
rownames(temp.ci)[ind_BCa_nt3]
#> [1] "Intercept" "X4.68499947" "X4.67499971" "X4.65499973" "X4.63499975"
#> [6] "X4.52499962" "X4.51499987" "X4.50499964" "X4.45499992" "X4.43499947"
#> [11] "X4.42499971" "X4.41499949" "X4.38499975" "X4.37499952" "X4.35499954"
#> [16] "X4.23499966" "X4.2249999" "X4.19499969" "X4.18499994" "X4.1449995"
#> [21] "X4.13499975" "X4.12499952" "X4.10499954" "X4.05499983" "X4.0449996"
#> [26] "X4.00499964" "X3.99499965" "X3.98499966" "X3.94499969" "X3.85499978"
#> [31] "X3.84499979" "X3.8349998" "X3.82499981" "X3.80499983" "X3.75499964"
#> [36] "X3.73499966" "X3.67499971" "X3.65499973" "X3.64499974" "X3.59499979"
#> [41] "X3.56499982" "X3.54499984" "X3.53499961" "X3.52499962" "X3.51499963"
#> [46] "X3.50499964" "X3.49499965" "X3.48499966" "X3.46499968" "X3.44499969"
#> [51] "X3.4349997" "X3.42499971" "X3.41499972" "X3.40499973" "X3.39499974"
#> [56] "X3.38499975" "X3.37499976" "X3.36499977" "X3.35499978" "X3.32499981"
#> [61] "X3.31499982" "X3.30499983" "X3.27499962" "X3.26499963" "X3.24499965"
#> [66] "X2.92499971" "X2.91499972"
```

`::plots.confints.bootpls(temp.ci,prednames=TRUE,indices = (2:nrow(temp.ci))[ind_BCa_nt3[-1]],articlestyle=FALSE) plsRglm`

`::plots.confints.bootpls(temp.ci,prednames=TRUE,indices = (2:nrow(temp.ci))[ind_BCa_nt3[-1]],articlestyle=FALSE,typeIC="BCa") plsRglm`

Finally, we fit a Beta PLS regression model constructed from the only 79 significant predictors for the \(BC_a\) bootstrap technique. The selection of the number of components, not reproduced here due to lack of space, leads us to retain 2 of them.

```
data("ind_BCa_nt3")
<- colon[c(TRUE,ind_BCa_nt3[-1])] colon_sub4
```

```
<- plsRbeta(X..Cellules.tumorales~.,data=colon_sub4,nt=10,modele="pls-beta")
modpls_sub4 #save(modpls_sub4,file="modpls_sub4.Rdata")
```

```
data("modpls_sub4")
modpls_sub4#> Number of required components:
#> [1] 10
#> Number of successfully computed components:
#> [1] 10
#> Coefficients:
#> [,1]
#> Intercept 1.5448748
#> X4.65499973 61.1773419
#> X4.6449995 306.0463140
#> X4.63499975 -284.4099752
#> X4.60499954 130.5717258
#> X4.59499979 20.9463141
#> X4.58499956 -281.2770768
#> X4.57499981 -144.3983544
#> X4.56499958 641.2859589
#> X4.55499983 942.5579964
#> X4.52499962 72.6143880
#> X4.51499987 -251.4256475
#> X4.50499964 -10.2039878
#> X4.43499947 -138.0766836
#> X4.42499971 -880.1560188
#> X4.38499975 -941.1056739
#> X4.37499952 -784.5708620
#> X4.35499954 -68.8364717
#> X4.2949996 109.1380862
#> X4.28499985 289.5456254
#> X4.27499962 -322.5710004
#> X4.26499987 71.6963047
#> X4.25499964 304.2135548
#> X4.20499992 481.8986709
#> X4.19499969 663.7076930
#> X4.18499994 79.9407749
#> X4.1449995 -27.1338795
#> X4.13499975 -0.6603306
#> X4.12499952 5.0923295
#> X4.11499977 -6.9928171
#> X4.10499954 7.3648246
#> X4.09499979 86.6345576
#> X4.05499983 -20.4373561
#> X4.0449996 -132.2956632
#> X4.00499964 -0.6487794
#> X3.99499965 -28.7700528
#> X3.98499966 -11.1008973
#> X3.85499978 201.1209855
#> X3.84499979 -112.9491585
#> X3.8349998 -112.0879668
#> X3.82499981 -176.4575677
#> X3.81499982 -499.4286464
#> X3.80499983 96.0999755
#> X3.7949996 -130.6062827
#> X3.76499963 2.2957805
#> X3.75499964 73.7212553
#> X3.73499966 110.5824104
#> X3.70499969 -9.6116865
#> X3.69499969 6.8740870
#> X3.66499972 -0.9445308
#> X3.65499973 7.7458993
#> X3.61499977 7.1671863
#> X3.59499979 -73.7235407
#> X3.56499982 -37.2007473
#> X3.54499984 27.1867459
#> X3.53499961 9.9491250
#> X3.52499962 -1.4676426
#> X3.51499963 -185.4622429
#> X3.50499964 201.8383573
#> X3.49499965 -390.4445054
#> X3.48499966 -141.9592003
#> X3.47499967 -134.0444094
#> X3.46499968 -141.0216380
#> X3.44499969 227.1789740
#> X3.4349997 -4.7983899
#> X3.42499971 -79.9664654
#> X3.41499972 26.0378859
#> X3.40499973 7.7258949
#> X3.37499976 -628.0508174
#> X3.36499977 -505.0750367
#> X3.35499978 140.4448861
#> X3.32499981 259.3811778
#> X3.31499982 -276.7503657
#> X3.30499983 491.7257390
#> X3.27499962 37.4180261
#> X3.26499963 28.6326050
#> X3.25499964 16.3911723
#> X3.24499965 37.8061066
#> X3.19499969 -32.7055621
#> X3.13499975 256.5034605
#> Information criteria and Fit statistics:
#> AIC BIC Chi2_Pearson_Y RSS_Y pseudo_R2_Y R2_Y
#> Nb_Comp_0 -32.34558 -27.58152 76.77937 6.4449550 NA NA
#> Nb_Comp_1 -129.57972 -122.43364 79.47275 2.2942689 0.7755421 0.6440210
#> Nb_Comp_2 -134.63789 -125.10979 72.20098 1.9728198 0.7991522 0.6938970
#> Nb_Comp_3 -142.69157 -130.78143 79.01884 1.8772302 0.8279202 0.7087287
#> Nb_Comp_4 -155.51742 -141.22526 84.08689 1.5340918 0.8558543 0.7619701
#> Nb_Comp_5 -164.67721 -148.00302 79.11247 1.3100325 0.8743807 0.7967352
#> Nb_Comp_6 -169.31896 -150.26274 75.81459 1.1376671 0.8794340 0.8234794
#> Nb_Comp_7 -171.85756 -150.41932 75.94769 1.1110661 0.8924862 0.8276068
#> Nb_Comp_8 -176.91620 -153.09593 71.83334 0.9526869 0.8974669 0.8521810
#> Nb_Comp_9 -186.16095 -159.95866 72.24187 0.8050430 0.9039681 0.8750894
#> Nb_Comp_10 -190.25562 -161.67130 71.24851 0.7212852 0.9063458 0.8880853
```

```
# Index plot
<- modpls_sub4$FinalModel;
sfit <- resid(sfit, type="sweighted2");
rd plot(seq(1,sfit$n), rd, xlab="Subject Index", ylab="Std Weigthed Resid 2",
main="Index Plot");
abline(h=0, lty=3);
```

We note on Figure 10 that this
model is validated by the use of a simulated envelope (Atkinson, 1981)^{43}. This
one is constructed, as recommended by Atkinson, from 19 order
statistics.

```
library(betareg)
# A Half-normal Plot with simulated envelop
<- predict(sfit, type="response");
phat <- predict(sfit, type="precision");
phihat <- modpls_sub4$tt
tt <- sfit$n
n <- list();
absrds for (i in 1:19)
{<- rbeta(sfit$n, phat*phihat, (1-phat)*phihat);
tYwotNA <- betareg(tYwotNA~tt, x=TRUE);
tsfit <- residuals(tsfit, type="sweighted2");
trd <-sort(abs(trd));
absrds[[i]]
}<- upper <- middle <- rep(0, n);
lower for (j in 1:n) {
<- max <- sum <- absrds[[1]][j];
min
min; max;for (i in 2:19) {
if (min > absrds[[i]][j]) min <- absrds[[i]][j];
if (max < absrds[[i]][j]) max <- absrds[[i]][j];
<- sum + absrds[[i]][j];
sum
}<- min;
lower[j] <- max;
upper[j] <- sum / 19;
middle[j]
}
<- qnorm((1:n+n-1/8) / (2*n+1/2)); qnval
```

```
plot(qnval, sort(abs(rd)), ylim=range(0,3.5), xlab="Expected", ylab="Observed",
main="Half-Normal Plot with Simulated Envelope");
lines(qnval, lower, lty=3);
lines(qnval, upper, lty=3);
lines(qnval, middle, lty=1, lwd=2);
```

As we can see in Figure 11, the two components of the model formed by the only 79 significant predictors for the bootstrap technique \(BC_a\) allow a clear linear separation between the healthy subjects, in dark green, and the sick subjects whose condition is all the more serious as the color approaches red. To allow a graphical evaluation of the predictive quality of the model, we represent the observed values as a function of the predicted values on the \(\textrm{logit}\) scale, Figure 12, and on the original scale Figure 13. Note that, since the transition from one to the other of the two graphs is done by means of a continuous, bijective and increasing transformation of the axes, the Kendall correlation coefficient between the observed and predicted values will be identical for both scales. For both graphs, we measure an association in the sense of Kendall’s correlation coefficient between the observed values and the predicted values equal to \(0.72\) for a \(p\)-value of \(7.03*10^{-19}\), thus significant at the \(1\%\) threshold.

`plot(modpls_sub4$tt[,1],modpls_sub4$tt[,2],col=plotrix::color.scale(colon[,1],c(0,1,1),c(1,1,0),0),pch=16,ylab="",xlab="")`

```
plot(binomial()$linkfun(modpls_sub4$ValsPredictY),binomial()$linkfun(orig[,1]),xlab="Valeurs prédites",ylab="Valeurs observées")
abline(0,1,lwd=2,lty=2)
```

```
plot(modpls_sub4$ValsPredictY,orig[,1],xlim=c(0,1),ylim=c(0,1))
abline(0,1,lwd=2,lty=2)
```

Note, however, that the data in this example are in fact formed by
mixing healthy individuals for whom the infiltration rate is zero and
sick individuals for whom these rates are non-zero and vary from 0 to 1.
Thus the use of a PLS regression model based on the combination of a
logistic PLS regression model and a Beta logistic regression model or a
Beta regression model with inflation of 0 introduced by Ospina and
Ferrari (Ospina, 2012)^{44} would likely make sense and is currently
being carried out by the authors.

## Conclusion and perspectives

Our goal has been to propose an extension of PLS regression to Beta
regression models, and then to make it available to users of the free
language `R`

.

We thus offer the possibility to work with collinear predictors to model rates or proportions, which is an unavoidable difficulty when modeling mixtures or when analyzing spectra or studying genetic, proteomic or metabonomic data.

Moreover, Beta PLS regression can also be applied to incomplete data sets. It is also possible in this case, as in the case of complete data, to select the number of components by repeated \(k\)-fold cross-validation.

Finally, we propose bootstrap techniques in order to, for example, test the significance of each of the predictors present in the dataset and thus validate the models built.

The study of two real datasets allowed the proposed tools to demonstrate their efficiency.

### Acknowledgements

The authors are grateful to the two referees for their comments and suggestions which helped to improve the quality of several points of the article as well as to Jean-Pierre Gauchi for his availability and his pertinent and constructive remarks.

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